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Population density, genetic diversity and hot spots of Commiphora wightii (Arnott) Bhandari in Rajasthan State
Journal of Applied Research on Medicinal and Aromatic Plants ( IF 3.8 ) Pub Date : 2021-06-06 , DOI: 10.1016/j.jarmap.2021.100323
Uttar Kumar Tomar , Hema Singhal , Arti Gaur , Lovelesh Singh Saini

Commiphora wightii (Arnott) Bhandari also known as ‘Guggal’, is a critically endangered medicinal plant species. Natural population of this species is declining due to poor regeneration, over-exploitation and destructive harvesting methods. In present study extensive surveys were conducted in all the guggal occurring forest areas of Rajasthan state with the objectives to study the population density, genetic diversity and its relationship with geographical patterns, distances and agroclimatic zones. Average population density of guggal was 21.9 plants ha−1 in guggal occurrence areas of forests in Rajasthan. The highest density (105.9 plants ha−1) was recorded in Sawai Madhopur and lowest (1.6 plants ha−1) in Jhalawar district. Variation in guggal population density was also observed due to altitude differences. The maximum density (40 plants ha−1) was recorded in altitude range from 301−350 m AMSL mainly in Aravalli range and it reduces at higher (>350 m AMSL) and lower (<301 m AMSL) altitudes.

Genetic diversity was studied with 96 genotypes collected from all over Rajasthan using selected six RAPD primers. The genetic distances among these genotypes ranged from 0.000 to 0.732. Statistical analysis indicated low genetic diversity (H = 0.195; I = 0.299), low genetic differentiation (GST = 0.169) and moderate gene flow (Nm = 2.455) between four agroclimatic zone populations. These results indicate that these populations are in process of genetic drift and genetic differentiation. The Mantel test showed statistically significant correlation (R = 0.306; p = 0.000) between pairwise geographical distance and genetic distance.



中文翻译:

拉贾斯坦邦Commiphora wightii ( Arnott) Bhandari 的种群密度、遗传多样性和热点

Commiphora wightii (Arnott) Bhandari 也被称为“Guggal”,是一种极度濒危的药用植物物种。由于再生不良、过度开发和破坏性的收获方法,该物种的自然种群正在下降。在本研究中,在拉贾斯坦邦所有古古尔森林地区进行了广泛的调查,目的是研究人口密度、遗传多样性及其与地理模式、距离和农业气候带的关系。在拉贾斯坦邦森林的 guggal 发生区,guggal 的平均种群密度为 21.9 株 ha -1。最高密度(105.9 株 ha -1)记录在 Sawai Madhopur 和最低(1.6 株 ha -1) 在贾拉瓦尔区。由于海拔差异,还观察到了古格尔人口密度的变化。最大密度(40 株 ha -1)记录在 301-350 m AMSL 的海拔范围内,主要在 Aravalli 范围内,并且在较高(>350 m AMSL)和较低(<301 m AMSL)海拔处降低。

使用选定的 6 种 RAPD 引物,对从拉贾斯坦邦各地收集的 96 种基因型进行了研究。这些基因型之间的遗传距离在 0.000 到 0.732 之间。统计分析表明 ,四个农业气候带种群之间的遗传多样性低(H  = 0.195;I  = 0.299)、遗传分化低(G ST  = 0.169)和中等基因流(N m = 2.455)。这些结果表明这些种群正处于遗传漂变和遗传分化过程中。Mantel 检验显示 成对地理距离和遗传距离之间存在统计学上的显着相关性(R  = 0.306;p = 0.000)。

更新日期:2021-06-09
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