Fully integrated uric acid (UA) and glucose biosensors were fabricated on polydimethylsiloxane/polyimide platform by facile one step laser scribed technique. The laser scribed graphene (LSG) on the thin polyimide film was functionalized using pyrenebutanoic acid, succinimide ester (PBSE) to improve the electrochemical activity of the biosensors. The LSG was further decorated with platinum nanoparticles (PtNPs) to promote the electrocatalytic activity towards the oxidation of UA. Glucose oxidase was immobilized on the PtNPs modified surface for selective detection of glucose. The fabricated biosensors were characterized via scanning electron microscopy (SEM), Energy dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS), and electrochemical methods (cyclic voltammetry and amperometry measurements). Outstanding electrocatalytic activities toward oxidation of UA and glucose were demonstrated. A wide detection range of 5 µM to 480 µM UA with a high sensitivity of 156.56 µA/mMcm² and a calculated detection limit (LOD) of 0.018 μM (S/N = 3) were achieved for the UA biosensor. The glucose biosensor exhibited a detection range of 5 µM to 3200 µM with a sensitivity of 12.64 µA/mMcm² and an LOD of 2.57 µM (S/N = 3). These integrated biosensors offer great promise for potential applications in wearable UA and glucose sensing due to their good sensitivity, selectivity, and stability properties.

完全集成的尿酸 (UA) 和葡萄糖生物传感器是在聚二甲基硅氧烷/聚酰亚胺平台上通过简便的一步激光划线技术制造的。使用芘丁酸、琥珀酰亚胺酯 (PBSE) 对聚酰亚胺薄膜上的激光划线石墨烯 (LSG) 进行功能化，以提高生物传感器的电化学活性。LSG 进一步用铂纳米粒子 (PtNPs) 装饰，以促进对 UA 氧化的电催化活性。葡萄糖氧化酶固定在 PtNPs 修饰的表面上，用于选择性检测葡萄糖。通过扫描电子显微镜 (SEM)、能量色散 X 射线 (EDX)、X 射线光电子能谱 (XPS) 和电化学方法（循环伏安法和电流法测量）对制造的生物传感器进行表征。证明了对 UA 和葡萄糖的氧化具有出色的电催化活性。5 µM 至 480 µM UA 的宽检测范围，具有 156.56 µA/mMcm 的高灵敏度UA 生物传感器达到了²和计算的检测限 (LOD) 为 0.018 μM (S/N = 3)。葡萄糖生物传感器的检测范围为 5 µM 至 3200 µM，灵敏度为 12.64 µA/mMcm ²，LOD 为 2.57 µM（S/N = 3）。由于其良好的灵敏度、选择性和稳定性，这些集成的生物传感器为可穿戴 UA 和葡萄糖传感中的潜在应用提供了广阔的前景。