Mononuclear phagocytes comprise an array of tissue-resident and monocyte-derived cells with important roles in tissue homeostasis and resistance to infection. Their diverse phenotypes make functional characterization within tissues challenging, because multiple surface markers are typically required for subset identification and isolation by cell sorting methods. Analysis of SLAMF9 expression within renal mononuclear phagocyte populations by multi-parametric flow cytometry indicates that SLAMF9 is a specific marker for identification of kidney-resident CD45⁺ CD11c⁺ MHC-II⁺ cells corresponding to prominent tissue-resident MPC populations derived from dendritic cell progenitors in adult mice. High SLAMF9 expression was sufficient to identify and sort these cells from disaggregated tissue using a user-operated cell sorter. The population can be further subdivided according to expression of CD11b and CD14 to identify IRF8^high cDC1 cells and cleanly separate the CD11b^high F4/80^low and CD11b^int F4/80^high CD11c⁺ MPC subsets. Therefore, SLAMF9 expression allows for the identification and sorting of kidney-resident CD11b⁺ CD11c⁺ CD64⁺ F4/80⁺ CX₃CR1⁺ MHC-II⁺ MPCs without the need for complex antibody panels or reporter mice, simplifying isolation of these cells for study ex vivo.

中文翻译：

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使用 SLAMF9 分离肾脏 MPC 的简化方法

单核吞噬细胞包含一系列组织驻留细胞和单核细胞衍生细胞，在组织稳态和抗感染中具有重要作用。它们多样化的表型使得组织内的功能表征具有挑战性，因为通过细胞分选方法进行子集识别和分离通常需要多个表面标记。通过多参数流式细胞术分析肾单核吞噬细胞群中的 SLAMF9 表达表明 SLAMF9 是鉴定肾脏驻留 CD45 ⁺ CD11c ⁺  MHC-II ^{+的特异性标志物}对应于来自成年小鼠树突状细胞祖细胞的显着组织常驻 MPC 种群的细胞。高 SLAMF9 表达足以使用用户操作的细胞分选仪从分解的组织中识别和分选这些细胞。人口可以根据 CD11b 和 CD14 的表达进一步细分，以识别 IRF8^高cDC1 细胞，并干净地分离 CD11b^高F4/80^低和 CD11b ^int F4/80^高CD11c ⁺ MPC 子集。因此，SLAMF9 表达允许识别和分类肾脏驻留 CD11b ⁺ CD11c ⁺ CD64 ⁺ F4/80 ⁺ CX₃ CR1 ⁺ MHC-II ⁺ MPC，无需复杂的抗体组或报告小鼠，简化了这些细胞的分离以进行离体研究。