An electrochemical sensor with high sensitivity and selectivity and excellent stability was developed for the determination of sofosbuvir (SOF), based on molecular imprinting strategy. The imprinted polymer was fabricated using acrylamide and methacrylic acid as monomers, 2, 2′-azobisisobutyronitrile (initiator), SOF as a template and ethylene glycol dimethacrylate (cross-linker). Fourier transform infrared spectroscopy, energy-dispersive X-ray and scanning electron microscopy were used for the characterization of the morphology and composition of the synthesized polymer. The proposed sensor was designed layer-by-layer assembled graphene oxide and imprinted polymer film on the surface of glassy carbon electrode. Cyclic voltammetry, square wave voltammetry and electrochemical impedance spectroscopy were utilized to investigate the electrochemical behavior of the modified sensor toward SOF. Under the optimum experimental condition, the relative current intensity of the sensor is directly proportional to the concentration of SOF in the range from 1 nM to 0.83 mM with lower detection limit of 0.013 nM. Moreover, the proposed sensor exhibited high selectivity (imprinting factor = 5.2), temporal stability (5 weeks), good reproducibility (RSD = 2.1%) and excellent sensitivity. Finally, the imprinted sensor was successfully applied for detection of SOF tracing in human blood of pre-treated patients and in sovaldi tablets with good recovery (98.5–100.7%).

基于分子印迹策略，开发了一种具有高灵敏度和选择性以及优异稳定性的电化学传感器，用于测定索非布韦（SOF）。使用丙烯酰胺和甲基丙烯酸作为单体、2, 2'-偶氮二异丁腈（引发剂）、SOF 作为模板和乙二醇二甲基丙烯酸酯（交联剂）制备印迹聚合物。傅里叶变换红外光谱、能量色散 X 射线和扫描电子显微镜用于表征合成聚合物的形态和组成。所提出的传感器是在玻碳电极表面逐层组装氧化石墨烯和印迹聚合物膜设计的。循环伏安法，方波伏安法和电化学阻抗谱被用来研究改性传感器对 SOF 的电化学行为。在最佳实验条件下，传感器的相对电流强度与 SOF 的浓度成正比，范围为 1 nM 至 0.83 mM，检测下限为 0.013 nM。此外，所提出的传感器具有高选择性（印记因子 = 5.2）、时间稳定性（5 周）、良好的重现性（RSD = 2.1%）和出色的灵敏度。最后，印迹传感器成功应用于检测预处理患者的人血和恢复良好的 sovaldi 片剂中的 SOF 示踪（98.5-100.7%）。传感器的相对电流强度与 SOF 的浓度成正比，范围为 1 nM 至 0.83 mM，检测下限为 0.013 nM。此外，所提出的传感器具有高选择性（印记因子 = 5.2）、时间稳定性（5 周）、良好的重现性（RSD = 2.1%）和出色的灵敏度。最后，印迹传感器成功应用于检测预处理患者的人血和恢复良好的 sovaldi 片剂中的 SOF 示踪（98.5-100.7%）。传感器的相对电流强度与 SOF 的浓度成正比，范围为 1 nM 至 0.83 mM，检测下限为 0.013 nM。此外，所提出的传感器具有高选择性（印记因子 = 5.2）、时间稳定性（5 周）、良好的重现性（RSD = 2.1%）和出色的灵敏度。最后，印迹传感器成功应用于检测预处理患者的人血和恢复良好的 sovaldi 片剂中的 SOF 示踪（98.5-100.7%）。