The ultimate success of micropropagation and the commercial viability of this technique depend not only on the number of regenerated seedlings but also on the ability to transfer them to an ex vitro environment at a large scale with high survival rates. In this context, the aim of this study was to achieve ex vitro establishment and to evaluate the morpho-anatomical changes and antioxidant enzyme activity that occurred during the acclimatization of Genipa americana L. seedlings. Shoot rooted in vitro were transferred to peat and vermiculite substrate and were maintained in a growth room under a controlled temperature of 25 ± 2 °C and a photosynthetic photon flux density of 67 μmol m⁻² s⁻¹. After 14 days of acclimatization in the growth room, the plants were transferred to a greenhouse with 70% shading. At 0 (corresponding to the in vitro phase), 7, 14, 21 and 28 days after the transfer to ex vitro conditions, growth and antioxidant enzyme activity were analyzed. After 30 days of acclimatization, 90% seedling survival was observed. The results reflect the high phenotypic plasticity of this species and morphological and physiological changes such as the emergence of new leaves, the increase in chlorophyll content, the stomata assuming an elliptical shape, the root survival in vitro and the stimulated activity of antioxidant enzymes, which ensured continuous plant growth and acclimatization, leading to continuous growth and a high survival rate (90%) after acclimatization.

微繁殖的最终成功和该技术的商业可行性不仅取决于再生幼苗的数量，还取决于将它们大规模转移到体外环境并具有高存活率的能力。在这种情况下，本研究的目的是实现体外建立并评估在美国格尼帕幼苗驯化过程中发生的形态解剖学变化和抗氧化酶活性。将体外生根的枝条转移到泥炭和蛭石基质中，并在控制温度为 25±2°C 和光合光子通量密度为 67 μmol m ^-2  s ^-1的生长室中维持. 在生长室驯化 14 天后，将植物转移到 70% 遮荫的温室中。在0（对应于体外阶段）、转移到体外条件后7、14、21和28天，分析生长和抗氧化酶活性。驯化 30 天后，观察到 90% 的幼苗成活率。结果反映了该物种表型可塑性高，出现新叶出现、叶绿素含量增加、气孔呈椭圆形、离体根系存活和抗氧化酶活性增强等形态和生理变化。保证植株连续生长和驯化，使驯化后持续生长，成活率高（90%）。