ABSTRACT

Objectives Adipocytes and adipocyte lipid metabolism are closely related with obesity and type 2 diabetes, but the molecular mechanism still needs further investigation. The aim of this study is to discover the adipocyte genes and pathways involved in obesity and type 2 diabetes using bioinformatics analysis.

Methods The GSE27951 gene expression profile was obtained. Software and online tools (STRING, Cytoscape, BioGPS, CTD, and FunRich) were used to identify core genes.21 human subcutaneous adipose samples, with 10 from type 2 diabetic patients and 11 from normal controls, were included in these analyses.

Results 184 differentially expressed genes (DEGs) including 42 up-regulated genes and 142 down-regulated genes were found to be enriched in metabolism, receptor activity, collagen type IV and glutamine biosynthesis I pathway by using the enrichment analysis. Seven hub genes were identified from the PPI network using various software (Cytoscape, STRING, BioGPS, and CTD). Four core genes (COL4A2, ACACB, GLUL, and CD36) were found to be highly expressed in subcutaneous adipose tissue of obese patients accompanying type 2 diabetes.

Conclusion COL4A2, ACACB, GLUL and CD36 might be the core molecular biomarkers of obesity in patients with or without type 2 diabetes.

摘要

目的脂肪细胞和脂肪细胞脂质代谢与肥胖和2型糖尿病密切相关，但其分子机制仍有待进一步研究。本研究的目的是利用生物信息学分析发现与肥胖和 2 型糖尿病相关的脂肪细胞基因和通路。

方法获得GSE27951基因表达谱。软件和在线工具（STRING、Cytoscape、BioGPS、CTD 和 FunRich）用于识别核心基因。这些分析包括 21 个人类皮下脂肪样本，其中 10 个来自 2 型糖尿病患者，11 个来自正常对照。

结果通过富集分析发现184个差异表达基因(DEGs)包括42个上调基因和142个下调基因在代谢、受体活性、IV型胶原和谷氨酰胺生物合成I途径中富集。使用各种软件（Cytoscape、STRING、BioGPS 和 CTD）从 PPI 网络中鉴定了七个中枢基因。发现四个核心基因（COL4A2、ACACB、GLUL 和 CD36）在伴有 2 型糖尿病的肥胖患者的皮下脂肪组织中高表达。

结论COL4A2、ACACB、GLUL和CD36可能是2型糖尿病患者或非2型糖尿病患者肥胖的核心分子标志物。