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Arsenic Accumulation in Hydroponically Grown Schizachyrium scoparium (Little Bluestem) Amended with Root-Colonizing Endophytes
ACS Earth and Space Chemistry ( IF 2.9 ) Pub Date : 2021-06-03 , DOI: 10.1021/acsearthspacechem.0c00302 Cherie L DeVore 1 , Eliane El Hayek 2 , Taylor Busch 3 , Benson Long 3 , Michael Mann 4 , Jennifer A Rudgers 4 , Abdul-Mehdi S Ali 5 , Tamara Howard 6 , Michael N Spilde 5 , Adrian Brearley 5 , Carlyle Ducheneaux 7 , Josée M Cerrato 3
ACS Earth and Space Chemistry ( IF 2.9 ) Pub Date : 2021-06-03 , DOI: 10.1021/acsearthspacechem.0c00302 Cherie L DeVore 1 , Eliane El Hayek 2 , Taylor Busch 3 , Benson Long 3 , Michael Mann 4 , Jennifer A Rudgers 4 , Abdul-Mehdi S Ali 5 , Tamara Howard 6 , Michael N Spilde 5 , Adrian Brearley 5 , Carlyle Ducheneaux 7 , Josée M Cerrato 3
Affiliation
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We integrated microscopy, spectroscopy, culturing and molecular biology, and aqueous chemistry techniques to evaluate arsenic (As) accumulation in hydroponically grown Schizachyrium scoparium inoculated with endophytic fungi. Schizachyrium scoparium grows in historically contaminated sediment in the Cheyenne River Watershed and was used for laboratory experiments with As(V) ranging from 0 to 2.5 mg L–1 at circumneutral pH. Arsenic accumulation in regional plants has been a community concern for several decades, yet mechanisms affecting As accumulation in plants associated with endophytic fungi remain poorly understood. Colonization of roots by endophytic fungi supported better external and vascular cellular structure, increased biomass production, increased root lengths and increased P uptake, compared to noninoculated plants (p value <0.05). After exposure to As(V), an 80% decrease of As was detected in solution and accumulated mainly in the roots (0.82–13.44 mg kg–1) of noninoculated plants. Endophytic fungi mediated intracellular uptake into root cells and translocation of As. Electron microprobe X-ray mapping analyses detected Ca–P and Mg–P minerals with As on the root surface of exposed plants, suggesting that these minerals could lead to As adsorption on the root surface through surface complexation or coprecipitation. Our findings provide new insights regarding biological and physical–chemical processes affecting As accumulation in plants for risk assessment applications and bioremediation strategies.
中文翻译：
水培栽培的Schizachyrium scoparium (Little Bluestem) 中砷的积累，并通过根部定植内生菌进行修正
我们整合了显微镜、光谱学、培养和分子生物学以及水性化学技术，以评估接种内生真菌的水培生长的Schizachyrium scoparium 中的砷 (As) 积累。Schizachyrium scoparium生长在夏延河流域历史上受污染的沉积物中，用于实验室实验，As(V) 范围为 0 至 2.5 mg L –1在中性 pH 值下。几十年来，区域植物中砷的积累一直是社区关注的问题，但影响与内生真菌相关的植物中砷积累的机制仍然知之甚少。与未接种的植物相比，内生真菌对根的定植支持更好的外部和维管细胞结构、增加的生物量产量、增加的根长度和增加的磷吸收（p值 <0.05）。暴露于 As(V) 后，在溶液中检测到的 As 减少了 80%，并且主要在根部积累（0.82–13.44 mg kg –1) 的未接种植物。内生真菌介导细胞内摄取到根细胞和 As 的易位。电子微探针 X 射线绘图分析在暴露植物的根表面检测到 Ca-P 和 Mg-P 矿物质与砷，表明这些矿物质可能通过表面络合或共沉淀导致根表面吸附砷。我们的发现为风险评估应用和生物修复策略提供了关于影响植物中砷积累的生物和物理化学过程的新见解。
更新日期：2021-06-17
中文翻译：
水培栽培的Schizachyrium scoparium (Little Bluestem) 中砷的积累，并通过根部定植内生菌进行修正
我们整合了显微镜、光谱学、培养和分子生物学以及水性化学技术，以评估接种内生真菌的水培生长的Schizachyrium scoparium 中的砷 (As) 积累。Schizachyrium scoparium生长在夏延河流域历史上受污染的沉积物中，用于实验室实验，As(V) 范围为 0 至 2.5 mg L –1在中性 pH 值下。几十年来，区域植物中砷的积累一直是社区关注的问题，但影响与内生真菌相关的植物中砷积累的机制仍然知之甚少。与未接种的植物相比，内生真菌对根的定植支持更好的外部和维管细胞结构、增加的生物量产量、增加的根长度和增加的磷吸收（p值 <0.05）。暴露于 As(V) 后，在溶液中检测到的 As 减少了 80%，并且主要在根部积累（0.82–13.44 mg kg –1) 的未接种植物。内生真菌介导细胞内摄取到根细胞和 As 的易位。电子微探针 X 射线绘图分析在暴露植物的根表面检测到 Ca-P 和 Mg-P 矿物质与砷，表明这些矿物质可能通过表面络合或共沉淀导致根表面吸附砷。我们的发现为风险评估应用和生物修复策略提供了关于影响植物中砷积累的生物和物理化学过程的新见解。
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