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Mitochondrial dynamics and degradation in the oleaginous yeast Lipomyces starkeyi
Genes to Cells ( IF 1.3 ) Pub Date : 2021-06-04 , DOI: 10.1111/gtc.12875
Lan Duan 1 , Koji Okamoto 1
Affiliation  

Emerging evidence implicates the vital role of mitochondria in lipid consumption and storage, highlighting the intimate link between energy production and saving. Although formation of giant lipid droplets, which is the key hallmark of the oleaginous yeast Lipomyces starkeyi, appears to be regulated in response to changes in mitochondrial shape and metabolism, technical limitations of genetic manipulation have become an obstacle to uncover the mitochondrial behavior in this nonconventional yeast. Here, we established an L. starkeyi strain stably expressing a fluorescent marker for monitoring mitochondrial morphology and degradation and found that mitochondria are mostly fragmented in L. starkeyi cells under fermentable, nonfermentable, and nitrogen depletion conditions. Notably, a fraction of mitochondria-specific fluorescent signals was localized to the vacuole, a lytic organelle in yeast, indicating degradation of mitochondria in those cells. This possible catabolic event was more predominant in cells under nutrient-poor conditions than that in cells under nutrient-rich conditions, concomitantly with lipid droplet formation. Collectively, our studies provide a new tool to investigate mitochondrial dynamics in L. starkeyi and decipher the potential role of mitochondrial degradation in lipid metabolism.

中文翻译:

产油酵母 Lipomyces starkeyi 的线粒体动力学和降解

新出现的证据表明线粒体在脂质消耗和储存中的重要作用,突出了能源生产和节约之间的密切联系。尽管作为产油酵母Lipomyces starkeyi的关键标志的巨大脂滴的形成似乎受到线粒体形状和代谢变化的调节,但基因操作的技术限制已成为揭示这种非常规线粒体行为的障碍酵母。在这里,我们建立了一个稳定表达荧光标记的L. starkeyi菌株,用于监测线粒体形态和降解,发现线粒体在L. starkeyi中大多是碎片化的可发酵、不可发酵和缺氮条件下的细胞。值得注意的是,线粒体特异性荧光信号的一小部分位于液泡中,液泡是酵母中的一种裂解细胞器,表明这些细胞中线粒体的降解。这种可能的分解代谢事件在营养贫乏条件下的细胞中比在营养丰富条件下的细胞中更为突出,伴随着脂滴的形成。总的来说,我们的研究提供了一种新的工具来研究L. starkeyi 的线粒体动力学并破译线粒体降解在脂质代谢中的潜在作用。
更新日期:2021-08-10
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