BACKGROUND:The Mexican serviceberry, Malacomeles denticulata, have been used as a successful oral therapy by Mexican communities without enough scientific support. OBJECTIVE:To evaluate the M. denticulata extracts with selective antibacterial properties over dental biofilm bacteria. METHODS:Fruit,Leaf, and Stem of M. denticulata extracts were evaluated with micro-broth dilution method using ATCC bacteria. OD600 values had compared against each positive control (T-student-test). Anaerobically viability had confirmed by Colony-Forming-Units. Thin-Layer-Chromatography was used to identify the number of compounds and phytochemicals to identify secondary metabolites of the selected extracts. RESULTS:Streptococcus mutans showed Minimum-Bactericidal-Concentrations_(MBC) at 30 mg/mL to Fruit, Leaf, and Stem extracts. Periodontal-pathogens Aggregatibacter actinomycetemcomitans serotype b_(MBC = 30 mg/mL_p < 0.01); Fusobacterium nucleatum subsp. nucleatum_(MBC = 30 mg/mL_p<0.001); Parvimonas micra_(MBC = 15 mg/mL_NS); Porphyromonas gingivalis_(MBC = 30 mg/mL_NS); and Prevotella intermedia_(MBC = 3.75 mg/mL_NS) presented higher sensitivity to Leaf-Methanol, than the primary colonizers. Phytochemicals showed positive results to anthraquinones, coumarins, flavonoids, saponins, saponins steroids/triterpenoids, steroids/triterpenes, and tannins/phenols. CONCLUSION:We suggest the natural extracts of fruit and leaf of the Mexican serviceberry for the preventive use over the oral cariogenic or periodontal biofilm species, by their selective antibacterial properties against pathogenic species evaluated in-vitro, and due to the presence of antibacterial secondary metabolites identified as flavonoids and saponins of M. denticulata leaf extracts.

中文翻译：

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墨西哥服务莓提取物对牙齿生物膜物种的体外抗菌特性

背景：墨西哥服务浆果 Malacomeles denticulata 已被墨西哥社区用作成功的口服疗法，但没有足够的科学支持。目的：评价齿状木虱提取物对牙齿生物膜细菌的选择性抗菌性能。方法：采用ATCC细菌微量肉汤稀释法，对小齿木兰提取物的果实、叶和茎进行评价。OD600 值已与每个阳性对照（T-student-test）进行比较。菌落形成单位证实了厌氧活力。薄层色谱法用于鉴定化合物和植物化学物质的数量，以鉴定所选提取物的次级代谢物。结果：变形链球菌对果实、叶和茎提取物的最低杀菌浓度_(MBC) 为 30 mg/mL。牙周病菌聚集放线菌血清型 b_(MBC = 30 mg/mL_p < 0.01); 具核梭杆菌亚种。成核_(MBC = 30 mg/mL_p<0.001); Parvimonas micra_(MBC = 15 mg/mL_NS); 牙龈卟啉单胞菌_(MBC = 30 mg/mL_NS); Prevotella intermedia_(MBC = 3.75 mg/mL_NS) 对叶甲醇的敏感性高于主要定植菌。植物化学物质对蒽醌、香豆素、黄酮类化合物、皂苷、皂苷类固醇/三萜、类固醇/三萜和单宁/酚类化合物显示出阳性结果。结论：我们建议墨西哥服务莓的果实和叶子的天然提取物用于预防口腔致龋或牙周生物膜物种，因为它们对体外评估的病原体物种的选择性抗菌特性，