Borage oil that is extracted from (Borago officinalis Linn.) is a well-known medicinal plant having various medicinal benefits. In this work, an affordable, simple, reliable, rapid and easily accessible high-performance thin-layer chromatography (HPTLC) method was developed for the estimation of gamma-linolenic acid (GLA) in borage oil. HPTLC method employs thin-layer chromatography (TLC) aluminum plates precoated with silica gel (G60F254) as the stationary phase, and the mixture of hexane:toulene:glacial acetic acid (3:7:1, v/v/v) was used as the mobile phase. Densitometric analysis of the TLC plates was carried out at 200 nm. The developed method showed well-resolved spots with retention factor (Rf) value of 0.53 ± 0.04 for GLA. Various experimental conditions like saturation time for chamber, solvent phase migration and width of the band were studied intensely for selecting the optimum conditions. The method validation was performed for parameters like linearity, accuracy, specificity and precision. The values of limit of detection and limit of quantification for GLA were found to be 0.221 and 0.737 μg/band, respectively. In nutshell, the developed HPTLC method was found to be highly sensitive for the estimation of GLA in the herbal oil samples and formulations.

中文翻译：

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一种经过验证、快速且经济高效的 HPTLC 方法，用于定量琉璃苣油中的 γ-亚麻酸并评估抗氧化活性

从 (Borago officinalis Linn.) 中提取的琉璃苣油是一种著名的药用植物，具有多种药用价值。在这项工作中，开发了一种经济实惠、简单、可靠、快速且易于获得的高性能薄层色谱 (HPTLC) 方法，用于估算琉璃苣油中的 γ-亚麻酸 (GLA)。HPTLC 方法采用预涂硅胶 (G60F254) 的薄层色谱 (TLC) 铝板作为固定相，使用己烷:甲苯:冰醋酸 (3:7:1, v/v/v) 的混合物作为流动相。TLC 板的光密度分析在 200 nm 处进行。所开发的方法显示 GLA 的保留因子 (Rf) 值为 0.53 ± 0.04 的很好分辨的斑点。各种实验条件，如室的饱和时间，为了选择最佳条件，对溶剂相迁移和谱带宽度进行了深入研究。对线性、准确度、特异性和精密度等参数进行了方法验证。GLA 的检测限和定量限分别为 0.221 和 0.737 μg/条带。简而言之，发现开发的 HPTLC 方法对估计草药油样品和配方中的 GLA 高度敏感。