Carlina acaulis L. can provide many valuable phytochemicals. However, the plant has a limited range and is under partial protection in many countries. Therefore, it is necessary to develop a cultivation methodology, including the mode of fertilisation. This study presents answers to the following research hypotheses: 1) C. acaulis plants can be successfully cultivated outside the natural habitat; 2) Nitrogen fertilisation of C. acaulis plants affects the yield of valuable metabolites. A cultivation experiment was carried out in a two-factor scheme with the random block method in five replicates. The research factors included the nitrogen dose (0, 4, and 8 g/m²) and the type of the nitrogen fertiliser, i.e. ammonium sulphate and calcium nitrate. The biomass yield and the metabolite content were determined with the gravimetric method and high performance liquid chromatography, respectively. It was found that C. acaulis plants were able to grow outside their natural habitat and nitrogen fertilisation negatively affected the yield of metabolites per plantation area. One hectare of cultivation without nitrogen fertilisation can provide approx. 18 kg of ursolic acid, 5 kg of oleanolic acid, 70 kg of chlorogenic acid, and 10 kg of carlina oxide. However, more research is needed to determine the effects of plant density, harvest time, and requirements for other nutrients.

Carlina acaulis L. 可以提供许多有价值的植物化学物质。然而，该植物的范围有限，在许多国家受到部分保护。因此，有必要开发一种栽培方法，包括施肥方式。本研究为以下研究假设提供了答案：1) C. acaulis植物可以在自然栖息地之外成功栽培；2) C. acaulis植物的氮肥影响有价值代谢物的产量。培养实验采用随机区组法在双因素方案中进行，重复 5 次。研究因素包括氮剂量（0、4 和 8 g/m ²) 和氮肥的类型，即硫酸铵和硝酸钙。生物质产量和代谢物含量分别用重量法和高效液相色谱法测定。发现C. acaulis植物能够在其自然栖息地之外生长，施氮对每个种植面积的代谢物产量产生负面影响。一公顷不施氮肥可提供约。18公斤熊果酸、5公斤齐墩果酸、70公斤绿原酸和10公斤氧化钆。然而，需要更多的研究来确定植物密度、收获时间和对其他营养物质的需求的影响。