Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics,STEM CELLS Translational Medicine

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Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics
STEM CELLS Translational Medicine ( IF 5.4 ) Pub Date : 2021-06-05 , DOI: 10.1002/sctm.15-0352
Sabrina C Lin ₁ , Antonio Loza ₁ , Lauren Antrim ₁ , Prue Talbot ₁

Affiliation

StemCellQC is a video bioinformatics software tool for the quantitative analysis of human pluripotent stem cell (hPSC) colonies. Our objective was to use StemCellQC to evaluate and compare various experimental culture conditions, cell lines, and treatments and to demonstrate its applicability to PSC problems. Seven key features were identified that provided useful information on PSC morphology, dynamic behavior, and viability. Colony attachment was better on laminin-521 than on Matrigel and Geltrex. Growth rates were similar on each matrix when data were normalized. The brightness/area ratio feature showed greater cell death in colonies grown on Matrigel and Geltrex than on laminin-521 further contributing to an overall greater yield of cells on laminin-521. Four different PSC culture media performed similarly; however, one medium produced batch-to-batch variation in colony morphology and dynamic features. Two embryonic and one induced pluripotent stem cell line showed significant differences in morphology, growth rates, motility, and death rates. Cells from the same vial that became phenotypically different in culture showed measurable differences in morphology, brightness, and motility. Likewise, differentiating and undifferentiated colonies varied in growth rate, intensity, and motility. Three pluripotent cell lines treated with a low concentration of cinnamaldehyde, a chemical used in consumer products, showed adverse effects and differed in their sensitivity to treatment. Our data demonstrate various applications of StemCellQC which could be used in basic and translational research, toxicological and drug testing, and clinical facilities engaged in stem cell therapy.

中文翻译：

人类多能干细胞形态、质量和细胞动力学的视频生物信息学分析

StemCellQC 是一种视频生物信息学软件工具，用于对人类多能干细胞 (hPSC) 菌落进行定量分析。我们的目标是使用 StemCellQC 来评估和比较各种实验培养条件、细胞系和处理方法，并证明其对 PSC 问题的适用性。确定了七个关键特征，它们提供了有关 PSC 形态、动态行为和生存能力的有用信息。层粘连蛋白 521 上的菌落附着优于 Matrigel 和 Geltrex。当数据归一化时，每个矩阵的增长率相似。亮度/面积比特征显示，在 Matrigel 和 Geltrex 上生长的菌落中的细胞死亡比在层粘连蛋白 521 上生长的菌落更多，这进一步有助于在层粘连蛋白 521 上总体上更高的细胞产量。四种不同的 PSC 培养基表现相似；然而，一种培养基产生了菌落形态和动态特征的批次间变化。两种胚胎干细胞系和一种诱导多能干细胞系在形态、生长速率、运动性和死亡率方面表现出显着差异。来自同一小瓶的细胞在培养中表现出不同的表型，在形态、亮度和运动性方面表现出可测量的差异。同样，分化和未分化的菌落在生长速度、强度和运动性方面也有所不同。用低浓度肉桂醛（一种用于消费品的化学物质）处理的三种多能细胞系显示出不良反应，并且它们对治疗的敏感性不同。我们的数据展示了 StemCellQC 的各种应用，可用于基础和转化研究、毒理学和药物测试，

更新日期：2021-06-05

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