The OsOXO2, OsOXO3 and OsOXO4 Positively Regulate Panicle Blast Resistance in Rice,Rice

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The OsOXO2, OsOXO3 and OsOXO4 Positively Regulate Panicle Blast Resistance in Rice
Rice ( IF 4.8 ) Pub Date : 2021-06-05 , DOI: 10.1186/s12284-021-00494-9
Jingfang Dong _{1,

2} , Lian Zhou _{1,

2} , Aiqing Feng ₃ , Shaohong Zhang _{1,

2} , Hua Fu _{1,

2} , Luo Chen _{1,

2} , Junliang Zhao _{1,

2} , Tifeng Yang _{1,

2} , Wu Yang _{1,

2} , Yamei Ma _{1,

2} , Jian Wang _{1,

2} , Xiaoyuan Zhu ₃ , Qing Liu _{1,

2} , Bin Liu _{1,

2}

Affiliation

Background

Although panicle blast is more destructive to yield loss than leaf blast in rice, the cloned genes that function in panicle blast resistance are still very limited and the molecular mechanisms underlying panicle blast resistance remain largely unknown.

Results

In the present study, we have confirmed that the three Oxalate oxidase (OXO) genes, OsOXO2, OsOXO3 and OsOXO4 from a blast-resistant cultivar BC10 function in panicle blast resistance in rice. The expression of OsOXO2, OsOXO3 and OsOXO4 were induced by panicle blast inoculation. Subcellular localization analysis revealed that the three OXO proteins are all localized in the nucleus and cytoplasm. Simultaneous silencing of OsOXO2, OsOXO3 and OsOXO4 decreased rice resistance to panicle blast, whereas the OsOXO2, OsOXO3 and OsOXO4 overexpression rice plants individually showed enhanced panicle blast resistance. More H₂O₂ and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants, while the silencing plants exhibited less H₂O₂ and lower expression levels of PR genes compared to the control plants. Moreover, phytohormone treatment and the phytohormone signaling related gene expression analysis showed that panicle blast resistance mediated by the three OXO genes was associated with the activation of JA and ABA signaling pathways but suppression of SA signaling pathway.

Conclusion

OsOXO2, OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice. The OXO genes could modulate the accumulation of H₂O₂ and expression levels of PR gene in plants. Moreover, the OXO genes mediated panicle blast resistance could be regulated by ABA, SA and JA, and may be associated with the activation of JA and ABA signaling pathways but suppression of the SA signaling pathway.

中文翻译：

OsOXO2、OsOXO3 和 OsOXO4 正向调控水稻穗瘟抗性

背景

尽管稻瘟病对水稻产量损失的破坏性大于叶瘟，但具有抗稻瘟病功能的克隆基因仍然非常有限，并且稻瘟病抗性的分子机制仍然很大程度上未知。

结果

在本研究中，我们已经证实来自抗稻瘟病品种 BC10的三个草酸氧化酶 ( OXO ) 基因OsOXO2、OsOXO3和OsOXO4在水稻穗抗稻瘟病中发挥作用。OsOXO2、OsOXO3和OsOXO4的表达受穗胚细胞接种诱导。亚细胞定位分析表明，三种OXO蛋白均定位于细胞核和细胞质中。同时沉默OsOXO2、OsOXO3和OsOXO4 会降低水稻对穗瘟的抗性，而OsOXO2、OsOXO3和OsOXO4过表达水稻植株个别表现出增强的穗稻瘟病抗性。与对照植物相比，在过表达植物中观察到更多的 H ₂ O ₂和更高的PR基因表达水平，而与对照植物相比，沉默植物表现出更少的 H ₂ O ₂和更低的PR基因表达水平。此外，植物激素处理和植物激素信号相关基因表达分析表明，三个OXO基因介导的穗瘟抗性与 JA 和 ABA 信号通路的激活有关，但与 SA 信号通路的抑制有关。