Tailored electrospun meshes have been increasingly explored for abdominal wall defect repair in preclinical and clinical studies. However, the fabrication of a bioengineered mesh adapts to the intraperitoneal repair for a compliant remodeling remains a great challenge. In this study, we fabricated a functional mesh by combining polycaprolactone (PCL) with silk fibroin (SF) and decellularized human amniotic membrane (HAM) proportionally via electrospinning. SF was integrated with PCL (40:60 w/w) to regulate the structural flexibility. Micronized HAM was incorporated to PCL/SF (10:90 w/w) to provide a biocompatible milieu with functions being conferred to facilitate intraperitoneal repair. After the blend electrospinning, the PCL/SF/HAM mesh was characterized in vitro and implanted into the rat model with a full-thickness defect for a comprehensive evaluation in comparison to the PCL and PCL/SF meshes. The results demonstrated that electrospinning fabricated PCL stabilized the mechanical elongation toward approximating the native counterparts after integrating with SF. After integrating with HAM, which is coupled with diverse biomolecular compositions, the developed PCL/SF/HAM mesh provided a better microenvironment for cell proliferation and vasculogenic network over other meshes without HAM addition and possessed the functions capable of inhibiting transforming growth factor β1 (TGF-β1) expression and collagen secretion under inflammatory conditions. Moreover, the functional mesh developed less-intensive adhesion along with histologically weaker inflammatory response and foreign body reaction than the PCL and PCL/SF meshes after 90 days in vivo. During the remodeling process, the bioactive structure induced more pronounced neovascularization and remarkable incorporation of collagen and elastin fibers and contractile filaments for a mechanically sufficient and physiologically stiffness-matched healing. This tailor-made mesh expands the intraperitoneal applicability of conventional electrospun meshes for a compliant remodeling in the repair of abdominal wall defects.

在临床前和临床研究中，越来越多地探索定制的静电纺丝网用于腹壁缺损修复。然而，生物工程网的制造适应腹膜内修复以进行顺应性重塑仍然是一个巨大的挑战。在这项研究中，我们通过静电纺丝按比例将聚己内酯 (PCL) 与丝素蛋白 (SF) 和脱细胞人羊膜 (HAM) 相结合，制造了功能性网格。SF 与 PCL (40:60 w/w) 集成以调节结构灵活性。微粉化 HAM 被纳入 PCL/SF (10:90 w/w) 以提供具有促进腹膜内修复功能的生物相容性环境。共混静电纺丝后，PCL/SF/HAM网在体外表征并植入具有全层缺损的大鼠模型，与 PCL 和 PCL/SF 网格进行比较进行综合评估。结果表明，在与 SF 结合后，静电纺丝制造的 PCL 使机械伸长率趋于接近天然对应物。与具有多种生物分子组成的 HAM 结合后，开发的 PCL/SF/HAM 网格为细胞增殖和血管生成网络提供了比未添加 HAM 的其他网格更好的微环境，并具有能够抑制转化生长因子 β1 (TGF) 的功能-β1) 在炎症条件下的表达和胶原蛋白分泌。而且，体内。在重塑过程中，生物活性结构诱导了更显着的新血管形成以及胶原蛋白和弹性蛋白纤维和收缩细丝的显着结合，以实现机械充分和生理刚度匹配的愈合。这种量身定制的网片扩大了传统电纺网片的腹膜内适用性，可在腹壁缺损修复中进行顺应性重塑。