To study the cyclooxygenase 2 (Cox 2) expression in newly diagnosed plasma cell myeloma cases by immunohistochemistry (IHC) and correlate with clinicohematological characteristics. Immunohistochemical expression of Cox 2 on bone marrow trephine biopsy was studied in seventy-three newly diagnosed myeloma cases [56 males, 17 females, median age; 58 years (36–75)] and fifteen controls using SP21 clone antibody. A median immuno-score (proportion x intensity) stratified the entire cohort into low and high expressors. Cox 2 immunoexpression was compared and correlated with clinicolaboratory characteristics and marrow histomorphology and survival. Twenty one of 73 (38.7%) cases had a plasmablastic morphology whereas remainder fifty-two (61.3%) had a differentiated morphology. The Cox 2 expression was noted in 71/73 (97.2%) cases (median score = 127.3) and 15/15 (100%) controls. Low expressors was associated with higher circulating plasma cells, increased marrow tumor burden, blastic morphology, and lower proliferation index (p < 0.05) with a peculiar ‘dot-block’ cytoplasmic positivity (p < 0.001); whereas high expressors had thinned out bony trabeculae with granular cytoplasmic positivity with or without membrane accentuation (p < 0.001). Cox 2 expression had a weak negative correlation with tumor burden (r; -0.32, p = 0.01) and positive correlation with proliferation index (r; 0.29, p = 0.03). There was no statistically significant difference in the survival between low (n = 20) and high (n = 23) expressors (log rank p = 0.11). A high proportion of myeloma cells in our cohort expressed Cox 2 using SP21 clone; and this may have a role in futuristic research and therapy.

通过免疫组织化学 (IHC) 研究新诊断的浆细胞骨髓瘤病例中环氧合酶 2 (Cox 2) 的表达及其与临床血液学特征的相关性。73例初诊骨髓瘤患者骨髓环钻活检Cox 2免疫组织化学表达研究[56 名男性，17 名女性，中位年龄；58 岁 (36–75)] 和 15 个使用 SP21 克隆抗体的对照。中位免疫评分（比例 x 强度）将整个队列分为低表达者和高表达者。比较了 Cox 2 免疫表达，并将其与临床实验室特征、骨髓组织形态学和存活率相关联。73 例中有 21 例 (38.7%) 具有浆母细胞形态，而其余 52 例 (61.3%) 具有分化形态。在 71/73 (97.2%) 个病例（中位数得分 = 127.3）和 15/15 (100%) 个对照中注意到 Cox 2 表达。低表达者与较高的循环浆细胞、增加的骨髓肿瘤负荷、成细胞形态和较低的增殖指数 ( p < 0.05)相关， 具有特殊的“点块”细胞质阳性 (p  < 0.001); 而高表达者的骨小梁变薄，细胞质呈颗粒状阳性，有或没有膜强化（p  < 0.001）。Cox 2 表达与肿瘤负荷呈弱负相关 (r; -0.32, p  = 0.01)，与增殖指数呈正相关 (r; 0.29, p  = 0.03)。低 ( n  = 20) 和高 ( n = 23) 表达者之间的存活率在统计学上没有显着差异 （log rank p  = 0.11）。我们队列中很大一部分骨髓瘤细胞使用 SP21 克隆表达 Cox 2；这可能在未来的研究和治疗中发挥作用。