Biofilm formation is an important physiological process in Staphylococcus aureus (S. aureus) that can cause infections in humans. In this study, the ability of 36 methicillin-resistant S. aureus (MRSA) clinical isolates to form biofilm was studied based on genotypic and phenotypic approaches. These isolates were genotyped based on the microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) and biofilm-associated genes (icaAD) via polymerase chain reactions. Phenotyping was performed based on the determination of the strength of biofilm formation of MRSA isolates in vitro. The most prevalent MSCRAMMs and biofilm-associated genes were clfA, eno, and icaD, followed by clfB. The fnbB (38.9%) and ebpS (11.1%) occurred less frequently among the MRSA isolates, while bbp and fnbA genes were absent from all isolates. The MRSA isolates were mostly moderate to strong biofilm formers, despite the heterogeneity of the MSCRAMM profiles. MRSA isolates from different infection sources (primary, catheter-related bloodstream, or secondary infections) were capable of forming strong biofilms. However, persistent bacteraemia was observed only in 19.4% of the MRSA-infected individuals. This study suggested that persistent MRSA bacteraemia in patients might not be associated with the biofilm-forming ability of the isolates.

生物膜形成是金黄色葡萄球菌( S. aureus )的重要生理过程，可引起人类感染。在本研究中，基于基因型和表型方法研究了 36株耐甲氧西林金黄色葡萄球菌(MRSA) 临床分离株形成生物膜的能力。这些分离株基于微生物表面成分通过聚合酶链反应识别粘附基质分子 (MSCRAMMs) 和生物膜相关基因 ( ica AD) 进行基因分型。基于体外 MRSA 分离物生物膜形成强度的测定进行表型分析。最普遍的 MSCRAMMs 和生物膜相关基因是clf A、eno和ica D，其次是clf B。fnb B (38.9%) 和ebp S (11.1%) 在 MRSA 分离株中出现频率较低，而bbp和fnb A 基因在所有分离株中均不存在。尽管 MSCRAMM 谱的异质性，MRSA 分离株大多是中度至强的生物膜形成剂。来自不同感染源（原发性、导管相关血流或继发性感染）的 MRSA 分离株能够形成强大的生物膜。然而，仅在 19.4% 的 MRSA 感染个体中观察到持续性菌血症。该研究表明，患者中持续存在的 MRSA 菌血症可能与分离物的生物膜形成能力无关。