Yarrowia lipolytica strains PGC01003 and PGC202 engineered for succinic acid production were studied and compared to the wild type strain W29. For the first time, these two strains were characterized in a chemically defined medium. Strain growth and organic acid production were investigated in fed-batch mode with glycerol as carbon and energy source. This study evaluated the impact of nitrogen deficiency strategy to redirect carbon flux toward succinic acid synthesis. Strain PGC01003 produced 19 g L⁻¹ succinic acid with an overall yield of 0.23 g g⁻¹ and an overall productivity of 0.23 g L⁻¹ h⁻¹, while strain PGC202 produced 33 g L⁻¹ succinic acid with an overall yield of 0.12 g g⁻¹ and a productivity of 0.57 g L⁻¹ h⁻¹. Nitrogen limitation effectively stopped biomass growth and increased succinic acid yield of PGC01003 and PGC202 by 18 % and 62 %, respectively. However, the specific succinic acid production rate was reduced by 77 % and 66 %, respectively.

研究了为生产琥珀酸而设计的解脂耶氏酵母菌株 PGC01003 和 PGC202，并与野生型菌株 W29 进行了比较。首次在化学成分确定的培养基中对这两种菌株进行了表征。以甘油为碳源和能源以分批补料模式研究菌株生长和有机酸生产。该研究评估了氮缺乏策略将碳通量重新导向琥珀酸合成的影响。菌株PGC01003产生19 g L ^-1琥珀酸，总产率为0.23 gg ^-1，总产率为0.23 g L ^-1 h ^-1，而菌株PGC202产生33 g L ^-1琥珀酸，总产率为0.12 gg ^-1和0.57 g L ^-1 h ^-1的生产率。氮限制有效地阻止了生物量生长，PGC01003 和 PGC202 的琥珀酸产量分别提高了 18% 和 62%。然而，特定的琥珀酸产率分别降低了 77% 和 66%。