Microbial fuel cell (MFC) has novel technological advances in simultaneous power generation and wastewater treatment applications. In this study, low-cost biosynthesized α-MnO₂ nanoparticles integration with conducting polyaniline (PANI) matrix to form α-MnO₂/PANI hybrid nanocomposite was fabricated by in situ polymerization method. The prepared material was characterized through UV-Vis spectroscopy, XRD, FTIR, TGA-DTA, DSC, SEM, cyclic voltammetry, and impedance spectroscopy. MFC performance study was done by using an external resistance in the range of 100 Ω–100 kΩ. The continuous test on bare pencil graphite electrode (PGE), α-MnO₂/PGE, PANI/PGE, and α-MnO₂/PANI/PGE were evaluated in glucose-fed-Escherichia coli-based MFC. It was found that α-MnO₂/PANI/PGE produces a maximum power and current densities of 426.26 ± 38.89 mW m⁻² and 2485.51 ± 397.31 mA m⁻², respectively. This was 6.5 and 5.7-fold higher in power and current densities than unmodified PGE. The maximum chemical oxygen demand produced by hybrid composite modified anode during closed circuit voltage or with external resistance and open circuit voltage (OCV) (circuit without connecting external resistance) measurements were found to be 88.19% and 92.27%, respectively. A maximum of 650.61 ± 10.11 mV OCV was obtained by α-MnO₂/PANI/PGE while 222.36 ± 8.16 mV of OCV was generated by PGE.

微生物燃料电池 (MFC) 在同步发电和废水处理应用方面具有新颖的技术进步。在这项研究中，低成本生物合成的α - MnO ₂纳米颗粒与导电聚苯胺（PANI）基质结合形成α- MnO ₂ /PANI 杂化纳米复合材料，采用原位聚合方法。通过紫外-可见光谱、XRD、FTIR、TGA-DTA、DSC、SEM、循环伏安法和阻抗谱对制备的材料进行了表征。MFC 性能研究是通过使用 100 Ω–100 kΩ 范围内的外部电阻来完成的。对裸铅笔石墨电极（PGE）、α- MnO ₂ /PGE、PANI/PGE和α- MnO ₂ /PANI/PGE 在葡萄糖喂养的基于大肠杆菌的 MFC 中进行评估。发现α- MnO ₂ /PANI/PGE 产生的最大功率和电流密度分别为426.26 ± 38.89 mW m ^-2和2485.51 ± 397.31 mA m ^-2。其功率和电流密度比未改性的 PGE 高 6.5 和 5.7 倍。发现在闭路电压或外部电阻和开路电压（OCV）（没有连接外部电阻的电路）测量期间，混合复合改性阳极产生的最大化学需氧量分别为 88.19% 和 92.27%。α- MnO获得的最大 650.61 ± 10.11 mV OCV₂ /PANI/PGE 而 222.36 ± 8.16 mV 的 OCV 是由 PGE 产生的。