For most people, the first step in treatment is to take out the tumor (surgery), so precise and fast diagnosis of any sign of high-risk and neoplastic cells, especially in surgical cavity margins, is significant. The frozen pathology method is the conventional standard of intraoperative diagnosis, but the low number of slides prepared from non-fixed tissues prevents us from achieving a perfect diagnosis. Although many improvements in intraoperative margin detection were achieved, still real-time detection of neoplastic lesions is crucial to improving diagnostic quality. Functionalized carbon nanotubes grown on the electrode needles lively and selectively determine the H₂O₂ released from cancer/atypical cells through reverse Warburg effect and hypoxia assisted glycolysis pathways in a quantitative electrochemical manner. The study was carried out on cell lines, 57 in vivo mice models with breast cancer, and 258 fresh in vitro samples of breast cancer tumors. A real-time electrotechnical system, named cancer diagnostic probe (CDP) (US Patent Pub. No.: US 2018/02991 A1, US 2021/0007638 A1, and US 2021/0022650 A1 [publications], and US 10,786,188 B1 [granted]), has been developed to find pre-neoplastic/neoplastic cells in vivo in a quantitative electrochemical manner by tracing hypoxia glycolysis byproducts. Matched pathological evaluations with response peaks of CDP were found based on the presence of neoplasia (from atypia to invasive carcinoma) in live breast tissues. The ability of CDP to find neoplastic lesions in mice models in vivo and fresh breast tumors in vitro was verified with sensitivity and specificity of 95% and 97%, respectively. The system may help a surgeon assistant system for usage in the operating room after passing many trials and standard examinations in the future.

中文翻译：

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碳纳米管传感器电化学追踪缺氧糖酵解，这是术中检测乳腺肿瘤可疑边缘的新标志


对于大多数人来说，治疗的第一步是切除肿瘤（手术），因此精确、快速地诊断任何高危细胞和肿瘤细胞的迹象，尤其是手术腔边缘的肿瘤细胞，非常重要。冰冻病理学方法是术中诊断的常规标准，但非固定组织制备的载玻片数量较少，导致我们无法实现完美的诊断。尽管术中切缘检测取得了许多改进，但肿瘤病变的实时检测对于提高诊断质量仍然至关重要。生长在电极针上的功能化碳纳米管通过逆Warburg效应和缺氧辅助糖酵解途径以定量电化学方式活跃并选择性地测定癌症/非典型细胞释放的H ₂ O ₂ 。该研究在细胞系、57 个体内乳腺癌小鼠模型和 258 个新鲜的体外乳腺癌肿瘤样本中进行。一种实时电工系统，名为癌症诊断探针（CDP）（美国专利公开号：US 2018/02991 A1、US 2021/0007638 A1、US 2021/0022650 A1 [出版物]，以及 US 10,786,188 B1 [授权] ]），已被开发用于通过追踪缺氧糖酵解副产物以定量电化学方式在体内寻找肿瘤前期/肿瘤细胞。根据活体乳腺组织中是否存在肿瘤（从异型性到浸润性癌），发现了与 CDP 响应峰相匹配的病理学评估。 CDP 在体内小鼠模型和体外新鲜乳腺肿瘤中发现肿瘤病变的能力经过验证，灵敏度和特异性分别为 95% 和 97%。 该系统未来经过多次试验和标准检查后，可以帮助外科医生辅助系统在手术室使用。