The effect of plastic additives on Shewanella oneidensis growth and function,Environmental Science: Processes & Impacts

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The effect of plastic additives on Shewanella oneidensis growth and function
Environmental Science: Processes & Impacts ( IF 4.3 ) Pub Date : 2021-5-27 , DOI: 10.1039/d1em00108f
Liam P Fawcett ₁ , Victoria S Fringer ₁ , Jessica R Sieber ₂ , Melissa A Maurer-Jones ₁

Affiliation

Plastic waste has the potential for significant consequences on various ecosystems; yet, there are gaps in our understanding of the interaction of bacteria with polymer additives. We studied the impact of representative additive molecules to the viability and cell function of Shewanella oneidensis MR-1. Specifically, we explored the toxicity of three bisphenols (bisphenol A (BPA), bisphenol S (BPS), and tetrabromo bisphenol A (TBBPA)) and two diesters (dibutyl sebacate (DBS) and diisobutyl phthalate (DIBP)) in order to evaluate the generalizability of toxicity based on similar molecular structures. TBBPA caused significant, dose-dependent decreases in viability for acute (4 h) exposures in aerobic and anaerobic conditions. While the other 4 additives showed no significant toxicity upon 4 h exposures, chronic (2 day) anaerobic exposures revealed a significant impact to growth. BPA and BPS cause a significant decrease in growth rates for all exposure doses (8–131 μM) while DBS and DIBP had decreases in growth for the lowest exposure concentrations, though recovered to growth rates similar to the control at the highest concentrations. This highlights that S. oneidensis may have the ability to use the diesters as a carbon source if present in high enough concentrations. Riboflavin secretion was monitored as a marker of cellular health. Most additives stimulated riboflavin secretion as a survival response. Yet, there was no generalizable trend observed for these molecules, indicating the importance of considering the nuances of molecular structure to toxicity responses and the need for further work to understand the consequences of plastic waste in our environment.

中文翻译：

塑料添加剂对 Shewanella oneidensis 生长和功能的影响

塑料垃圾有可能对各种生态系统产生重大影响；然而，我们对细菌与聚合物添加剂相互作用的理解存在差距。我们研究了代表性添加剂分子对Shewanella oneidensis活力和细胞功能的影响MR-1。具体而言，我们探讨了三种双酚（双酚 A (BPA)、双酚 S (BPS) 和四溴双酚 A (TBBPA)）和两种二酯（癸二酸二丁酯 (DBS) 和邻苯二甲酸二异丁酯 (DIBP)）的毒性，以评估基于相似分子结构的毒性的普遍性。TBBPA 在需氧和厌氧条件下导致急性（4 小时）暴露的生存能力显着下降，呈剂量依赖性。虽然其他 4 种添加剂在 4 小时暴露后没有表现出明显的毒性，但长期（2 天）厌氧暴露对生长有显着影响。BPA 和 BPS 导致所有暴露剂量 (8–131 μM) 的生长速率显着下降，而 DBS 和 DIBP 在最低暴露浓度下生长速度下降，但在最高浓度下恢复到与对照相似的生长速率。如果存在足够高的浓度，S. oneidensis可能有能力使用二酯作为碳源。监测核黄素分泌作为细胞健康的标志。大多数添加剂刺激核黄素分泌作为生存反应。然而，没有观察到这些分子的普遍趋势，这表明考虑分子结构对毒性反应的细微差别的重要性，以及需要进一步工作以了解塑料废物对我们环境的影响。

更新日期：2021-06-04

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