Objectives. Soft denture liners provide a favorable environment for adhesion and colonization of microorganisms. This in vitro study aimed to examine the efficacy of different concentrations of copper oxide nanoparticles (CuO NPs) incorporation into soft denture liner on the biofilm formation of the microbial species. Methods. Field Emission Scanning Electron Microscopy (FESEM) images from NPs were recorded. Antifungal susceptibility testing of CuO NPs against five standard strains of Candida albicans (CBS 10261, 1905, 1912, 1949, 2730), Streptococcus mutans (ATCC35668), Streptococcus sobrinus (ATCC27607), and Streptococcus salivarius (ATCC9222) was performed by the broth microdilution method with the Clinical and Laboratory Standards Institute reference method. The biofilm inhibition percentages of CuO NPs on the soft denture liners were determined by XTT assay. Results. The characterization of CuO NPs by scanning electron microscope (SEM) analyses confirmed the synthesis of NPs with appropriate structure and size with a mean diameter of 18.3 ± 9.1 nm. The CuO NPs successfully inhibited the growth of the tested standard strains of C. albicans and Streptococcus spp. at concentrations ranging from 64 to 128 µg mL⁻¹. Indeed, incorporation of CuO NPs at a concentration of 500 µg mL⁻¹ into the soft denture liners exhibited a significant activity (75%) in inhibition of C. albicans. biofilm formation in a dose-dependent manner. The biofilm formation of C. albicans in the presence of CuO NPs was lower than Streptococcus spp. in comparison with the control group (). Conclusion. Incorporation of CuO NPs significantly decreased the colonization and plaque formation of the oral pathogens, especially C. albicans accumulation. These NPs may be useful as a promising agent for the antimicrobial management of soft denture liner materials.

中文翻译：

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软义齿衬垫内氧化铜纳米颗粒对口腔病原体的抗菌和抗菌膜活性评估


目标。柔软的义齿衬垫为微生物的粘附和定植提供了有利的环境。这项体外研究旨在检验不同浓度的氧化铜纳米颗粒 (CuO NP) 掺入软义齿衬垫中对微生物物种生物膜形成的功效。方法。记录来自 NP 的场发射扫描电子显微镜 (FESEM) 图像。 CuO NPs 对白色念珠菌(CBS 10261、1905、1912、1949、2730)、变形链球菌(ATCC35668)、远缘链球菌(ATCC27607) 和唾液链球菌 ( ATCC9222) 的五种标准菌株进行了抗真菌敏感性测试肉汤微量稀释方法采用临床和实验室标准研究所参考方法。通过 XTT 测定测定软义齿衬垫上 CuO NP 的生物膜抑制百分比。结果。通过扫描电子显微镜 (SEM) 分析对 CuO 纳米颗粒进行表征，证实了纳米颗粒的合成具有适当的结构和尺寸，平均直径为 18.3 ± 9.1 nm。 CuO NPs 成功抑制了所测试的白色念珠菌和链球菌标准菌株的生长。浓度范围为 64 至 128 µg mL ^-1 。事实上，将浓度为 500 µ g mL ^-1的 CuO NP 掺入软义齿衬垫中，在抑制白色念珠菌方面表现出显着的活性 (75%)。生物膜的形成呈剂量依赖性。在 CuO NP 存在下，白色念珠菌的生物膜形成低于链球菌属。 与对照组相比（ ）。结论。 CuO NPs 的掺入显着减少了口腔病原体的定植和菌斑形成，尤其是白色念珠菌的积累。这些纳米颗粒可作为一种有前途的软义齿衬垫材料抗菌管理剂。