In this study, we analysed the intracellular fatty acid profiles of Komagataella phaffii during methylotrophic growth. K. phaffii grown on methanol had significantly lower total fatty acid contents in the cells compared with glucose-grown cells. C18 and C16 fatty acids were the predominant fatty acids in K. phaffii, although the contents of odd-chain fatty acids such as C17 fatty acids were also relatively high. Moreover, the intracellular fatty acid composition of K. phaffii changed in response to not only carbon sources but also methanol concentrations: C17 fatty acids and C18:2 content increased significantly as methanol concentration increased, whereas C18:1 and C18:3 contents were significantly lower in methanol-grown cells. The intracellular content of unidentified compounds (C_nH_2nO₄), on the other hand, was significantly greater in cells grown on methanol. As the intracellular contents of these C_nH_2nO₄ compounds were significantly higher in a gene-disrupted strain for glutathione peroxidase (gpx1Δ) than in the wild-type strain, we presume that the C_nH_2nO₄ compounds are fatty acid peroxides. These results indicate that K. phaffii can coordinate intracellular fatty acid composition during methylotrophic growth in order to adapt to high-methanol conditions and that certain fatty acid species such as C17:0, C17:1, C17:2 and C18:2 may be related to the physiological functions by which K. phaffii adapts to high-methanol conditions.

中文翻译：

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在低和高甲醇条件下生长的甲基营养酵母 Komagataella phaffii 的脂肪酸组成

在这项研究中，我们分析了Komagataella phaffii在甲基营养生长过程中的细胞内脂肪酸谱。与葡萄糖生长的细胞相比，在甲醇上生长的K. phaffii细胞中的总脂肪酸含量显着降低。C18和C16脂肪酸是法菲克氏杆菌中的主要脂肪酸，但C17脂肪酸等奇数链脂肪酸的含量也较高。此外， K. phaffii的细胞内脂肪酸组成不仅响应碳源而且甲醇浓度发生变化：C17 脂肪酸和 C18:2 含量随着甲醇浓度的增加而显着增加，而 C18:1 和 C18:3 含量在甲醇生长的细胞中显着降低。另一方面，在甲醇上生长的细胞中，不明化合物 (C _n H _2n O _{4 ) 的细胞内含量明显更高。}由于这些 C _n H _2n O _{4化合物的细胞内含量在谷胱甘肽过氧化物酶 (} gpx1 Δ)基因破坏菌株中显着高于野生型菌株，我们推测 C _n H _2n O ₄化合物是脂肪酸过氧化物。这些结果表明，K. phaffii可以在甲基营养生长期间协调细胞内脂肪酸组成，以适应高甲醇条件，并且某些脂肪酸种类如 C17:0、C17:1、C17:2 和 C18:2 可能是与K. phaffii适应高甲醇条件的生理功能有关。