Natural Deep Eutectic Solvents (NADES) are composed of supramolecular interactions of two or more natural compounds, such as organic acids, sugars, and amino acids, and they are being used as a new media alternative to conventional solvents. In this study, a new application of NADES is presented as a possible technology for biofilm structural breaker in complex systems since the current solvents used for biofilm cleaning and extraction of biofilm components use hazardous solutions. The NADES (betaine:urea:water and lactic acid:glucose:water) were analyzed before and after the biofilm treatment by attenuated total reflection Fourier-transform infrared spectroscopy and fluorescence excitation-emission matrix spectroscopy. Our results indicate that the green solvents could solubilize up to ≈70 percent of the main components of the biofilms extracellular matrix. The solubilization of the biomolecules weakened the biofilm structure, which could enhance the biofilm solubilization and removal. The NADES have the potential to be an environment-friendly, green solvent to extract valuable compounds and break the main structure from the biofilm, leading to a greener method for extracellular polymeric substance (EPS) extraction and biofilm treatment in various water treatment systems.

天然深共晶溶剂 (NADES) 由两种或多种天然化合物（如有机酸、糖和氨基酸）的超分子相互作用组成，它们正被用作替代传统溶剂的新介质。在这项研究中，由于当前用于生物膜清洁和生物膜成分提取的溶剂使用危险溶液，因此 NADES 的新应用被提出作为复杂系统中生物膜结构破坏的可能技术。NADES（甜菜碱：尿素：水和乳酸：葡萄糖：水）在生物膜处理前后通过衰减全反射傅里叶变换红外光谱和荧光激发发射矩阵光谱进行分析。我们的结果表明，绿色溶剂可以溶解高达 ≈70% 的生物膜细胞外基质的主要成分。生物分子的溶解削弱了生物膜结构，这可以增强生物膜的溶解和去除。NADES 有可能成为一种环境友好的绿色溶剂，可以提取有价值的化合物并破坏生物膜的主要结构，从而为各种水处理系统中的细胞外聚合物 (EPS) 提取和生物膜处理提供一种更环保的方法。