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Identification of a novel mutant allele, fzp-15, involved in panicle branch pattern of rice (Oryza sativa)
Plant Breeding ( IF 1.5 ) Pub Date : 2021-06-03 , DOI: 10.1111/pbr.12928
Haiyuan Chen 1 , Yue Cai 2 , Suobing Zhang 1 , Weijie Tang 1 , Xianwen Fang 1 , Yunhui Zhang 1, 3 , Toshio Yamamotob
Affiliation  

FRIZZY PANICLE (FZP) is involved in the development of panicle branching in rice. Herein, a novel mutant fzp-15, demonstrating the sequential rounds of branching and severe defects in SM (spikelet meristem) formation, was identified. Map-based cloning revealed that the abnormal panicle architecture of fzp-15 resulted from an amino acid substitution (Glu (E) to Ala (A)) in the N-terminal ERF domain of rice FZP protein. To generate new advantageous FZP alleles increasing grain numbers, CRISPR/Cas9 multiplex editing system was used to target the 5’ untranslation region (UTR) of FZP in 'Nipponbare' (NIP). The 85-bp (−156 to -72 bp) or 149-bp (−157 to -9 bp) deletion in the upstream region of FZP can significantly increase grain numbers without a decrease in grain size, while the 38-bp deletion (−44 to −7 bp) in the upstream region of FZP has little effect in changing grain numbers. These results indicated that the 113-bp fragment (−157 to −45 bp) upstream of FZP is important to its function and deletion of this region can be used to increase grain numbers in rice.

中文翻译:

鉴定一种新的突变等位基因 fzp-15,涉及水稻(Oryza sativa)的穗分枝模式

FRIZZY PANICLE ( FZP ) 参与水稻穗分枝的发育。在此,鉴定了一种新的突变体fzp-15,其证明了 SM(小穗分生组织)形成中的分枝和严重缺陷的连续轮次。基于图谱的克隆表明,fzp-15的异常穗结构是由水稻 FZP 蛋白 N 端 ERF 结构域中的氨基酸取代(Glu (E) 到 Ala (A))引起的。为了产生增加谷物数量的新的有利 FZP等位基因,使用 CRISPR/Cas9 多重编辑系统靶向“日本晴”(NIP)中FZP的 5' 非翻译区(UTR )。上游区域的 85-bp(-156 至 -72 bp)或 149-bp(-157 至 -9 bp)缺失FZP可以显着增加粒数而不减小粒数,而FZP上游区域的 38-bp 缺失(-44 至 -7 bp)对改变粒数影响不大。这些结果表明FZP上游的 113 bp 片段(-157 至 -45 bp)对其功能很重要,该区域的缺失可用于增加水稻的籽粒数。
更新日期:2021-08-07
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