The aim of the study was to investigate specific potential markers for cells obtained from three layers of human AAA divided into three segments along the AAA based on morphological differences. The isolated cells were compared to control commercial cell types from healthy human abdominal aortas. For each type of aortic layer, three specimens from 6 patients were compared. Total RNA was isolated from 36 cell cultures for gene expression profiling and potential new cytometry markers were typed. Isolated cells were analyzed by flow cytometry by using fluorochrome-conjugated antibodies to markers: CNN1, MYH10, ENG, ICAM2, and TEK. The relative expression of 45 genes in primary cell cultures and control lines was analyzed. Statistically significant differences were found in the expression of most of the analyzed genes between individual layers and control lines. Based on relative expression, antibodies were selected for flow cytometry. Gene expression profiles allowed to select new potential cytometry markers: CNN1, MYH10, MYOCD, ENG, ICAM2, TEK. However, none of the tested markers seems to be optimal and characteristic for a specific layer of AAA.

该研究的目的是研究从三层人类 AAA 中获得的细胞的特定潜在标记，这些细胞根据形态差异沿 AAA 分为三个部分。将分离的细胞与来自健康人腹主动脉的对照商业细胞类型进行比较。对于每种类型的主动脉层，比较了来自 6 名患者的三个样本。从 36 个细胞培养物中分离总 RNA 用于基因表达谱分析，并对潜在的新细胞计数标记进行分型。通过流式细胞术分析分离的细胞，使用荧光染料偶联的标记抗体：CNN1、MYH10、ENG、ICAM2 和 TEK。分析了45个基因在原代细胞培养物和对照系中的相对表达。在各个层和对照系之间的大多数分析基因的表达中发现了统计学上的显着差异。基于相对表达，选择抗体用于流式细胞术。基因表达谱允许选择新的潜在细胞计数标记：CNN1、MYH10、MYOCD、ENG、ICAM2、TEK。然而，对于特定的 AAA 层，没有一个测试标记似乎是最佳的和特征。