Abstract

Background: The human bocavirus (HBoV) is associated with gastrointestinal infections and was shown to persist in lung and colorectal tumors. Novel data suggest that HBoV may induce chronic inflammatory disease that in turn may lead to tumor development. Thereby it remains unknown if chronic inflammatory diseases like Morbus Crohn and Ulcerative colitis go ahead with a higher prevalence of the virus compared to control cohorts. Methods: formalin fixed paraffin embedded tissue samples from adult patients and children and two control groups were tested for the presence of HBoV DNA with a PCR that was designed to determine between the four known HBoV species. Results: While there was no statistical significance between the pediatric HBoV and control cohorts, there was a significant higher prevalence of HBoV occurrence in adult patients with Crohn and Ulcerative colitis. Discussion: The present study provides evidence for the persistence of the human bocavirus in patients with Morbus Crohn and Ulcerative colitis. With statistical significance the virus was more frequently detected in tissues of the Morbus Crohn and Ulcerative colitis patient cohorts than in the control cohorts. Conclusion: The data give raise to the hypothesis that HBoV could be a trigger of acute exacerbations of these diseases or may be one cause of the diseases.

中文翻译：

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人类博卡病毒持续存在与克罗恩病和溃疡性结肠炎相关的证据

摘要

背景：人类博卡病毒 (HBoV) 与胃肠道感染有关，并被证明在肺和结直肠肿瘤中持续存在。新数据表明，HBoV 可能诱发慢性炎症性疾病，进而可能导致肿瘤发展。因此，与对照组相比，慢性炎症性疾病（如克罗恩病和溃疡性结肠炎）是否会以更高的病毒流行率继续发展仍是未知数。方法：使用旨在确定四种已知 HBoV 物种之间的 PCR 检测来自成人患者和儿童以及两个对照组的福尔马林固定石蜡包埋组织样本中是否存在 HBoV DNA。结果：虽然儿科 HBoV 和对照组之间没有统计学意义，但成人克罗恩和溃疡性结肠炎患者的 HBoV 发生率显着更高。讨论：本研究为克罗恩病和溃疡性结肠炎患者中人博卡病毒的持续存在提供了证据。具有统计显着性的病毒在 Morbus Crohn 和溃疡性结肠炎患者群组的组织中比在对照群组中更频繁地检测到。结论：这些数据提出了一个假设，即 HBoV 可能是这些疾病急性加重的触发因素，或者可能是这些疾病的一个原因。