Numerous studies have reported that gravity alteration displays a remarkable influence on the biological processes of cancer cells. Therefore, gravity-related experiments have become a promising method of improving knowledge in the field of cancer biology and may be useful to detect remarkable implications for future cancer treatment. Taking this concept further, we used a 3D clinostat (3D-C; 10 rpm of changing direction) to analyse the effect of short-term exposure to simulated microgravity (sμg) on cisplatin sensitivity of drug resistant human ovarian cancer cells SKOV-3. This allowed us to investigate whether altered gravity affects drug susceptibility of cancer cells. Our studies revealed that sμg exposure affects SKOV-3 cells morphology and drug efficiency. We observed the altered cell shape, the presence of membrane blebbing and lamellipodia as well as the lack of filopodia when the cells had been cultured on 3D-C for 2 h. Cytotoxicity, cell death and cell cycle assays showed an increased percentage of apoptotic cells and G0/G1 cell cycle arrest after exposure on the 3D-C with cisplatin in comparison to the static control, non clinorotated cells. Cell proliferation and migration were altered after the exposure to sμg as well. Our studies suggest that the altered gravity conditions affected cellular mechanisms involved in cisplatin resistance, resulting in higher sensitivity of cancer cells to the chemotherapeutic. The investigation and clarification of these results may be a crucial step toward improving our understanding of the relationship between cellular resistance to chemotherapy and the response to altered gravitational conditions.

许多研究报告称，重力改变对癌细胞的生物学过程具有显着影响。因此，重力相关实验已成为提高癌症生物学领域知识的一种有前途的方法，并且可能有助于检测对未来癌症治疗的显着影响。进一步考虑这个概念，我们使用 3D 斜立仪（3D-C；10 rpm 改变方向）来分析短期暴露于模拟微重力 ( sμg ) 对耐药人卵巢癌细胞 SKOV-3 顺铂敏感性的影响。这使我们能够研究改变的重力是否会影响癌细胞的药物敏感性。我们的研究表明，sμg暴露影响 SKOV-3 细胞形态和药物效率。当细胞在 3D-C 上培养 2 小时时，我们观察到细胞形状的改变、膜起泡和片状伪足的存在以及丝状伪足的缺乏。细胞毒性、细胞死亡和细胞周期测定显示，与静态对照、非斜旋细胞相比，在 3D-C 上暴露于顺铂后，凋亡细胞和 G0/G1 细胞周期停滞的百分比增加。暴露于sμg后细胞增殖和迁移发生改变以及。我们的研究表明，改变的重力条件影响了参与顺铂耐药的细胞机制，导致癌细胞对化疗药物的敏感性更高。对这些结果的调查和澄清可能是提高我们对细胞对化疗的抗性与对改变的重力条件的反应之间关系的理解的关键一步。