The transmission spectra of femtosecond laser pulses in the mid-IR range (5–6.6 μm) were investigated of submonolayer of Staphylococcus aureus and Pseudomonas aeruginosa bacteria on a silicon plate in the region of characteristic absorption bands of proteins and lipids, as well as stationary spectra after irradiation. Invertible “translucence” of samples in the region of characteristic bands of bacteria and blue shift these bands indicating the destruction of hydrogen bonds were discovered. The possibility of inactivation of pathogenic bacteria by selective IR laser denaturation of functional proteins by resonance irradiation of low average power is discussed.

中文翻译：

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6 µm 光谱范围内细菌中特征分子振动的飞秒红外激光光谱

飞秒激光脉冲在中红外范围 (5-6.6 μm) 的透射光谱研究了硅板上金黄色葡萄球菌和铜绿假单胞菌细菌在蛋白质和脂质特征吸收带区域以及静止的亚单层辐照后的光谱。发现细菌特征带区域中样品的可逆“半透明”和蓝移，这些带表明氢键被破坏。讨论了通过低平均功率的共振照射使功能蛋白选择性红外激光变性灭活病原菌的可能性。