Applying different spectroscopic techniques for the selective determination of daclatasvir using merbromin as a probe: Applications on pharmaceutical analysis,Luminescence

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Applying different spectroscopic techniques for the selective determination of daclatasvir using merbromin as a probe: Applications on pharmaceutical analysis
Luminescence ( IF 3.2 ) Pub Date : 2021-06-03 , DOI: 10.1002/bio.4099
Albandary Almahri ₁ , Mohamed A Abdel-Lateef ₂

Affiliation

In this study, rapid resonance Rayleigh scattering (RRS), spectrophotometric, and spectrofluorimetric methods were performed for facile quantitation of daclatasvir dihydrochloride without interference from sofosbuvir (a co-formulated anti-hepatitis C virus drug). The proposed approaches were based on forming a binary complex between daclatasvir dihydrochloride and merbromin reagent at pH 4.1. The binary complex was measured spectrophotometrically at λ_max = 544 nm. The spectrofluorimetric approach relied on the quenching effect of daclatasvir dihydrochloride on the fluorescence strength of merbromin at λ_Emission = 545 nm. The RRS approach depended on augmentation in the merbromin RRS spectrum at 363 nm upon addition of daclatasvir dihydrochloride. The presented methodologies were linear over the concentration ranges 2.5−15.0, 0.2−1.6 and 0.15−3.0 μg ml⁻¹ with detection limits of 0.45, 0.046, and 0.036 μg ml⁻¹ for the spectrophotometric approach, the spectrofluorometric approach, and RRS approach, respectively. Current approaches were validated in compliance with International Council for Harmonisation guidelines and utilized practically to estimate daclatasvir dihydrochloride either in binary mixtures with sofosbuvir or in its commercial tablet dosage form with good results. Moreover, the test for content uniformity was applied successfully on commercial tablets using the current spectroscopic approaches.

中文翻译：

应用不同的光谱技术以 merbromin 作为探针选择性测定达卡他韦：在药物分析中的应用

在本研究中，使用快速共振瑞利散射 (RRS)、分光光度法和荧光分光光度法对达卡他韦二盐酸盐进行了简便的定量，而不受索非布韦（一种共同配制的抗丙型肝炎病毒药物）的干扰。所提出的方法基于在 pH 4.1 下形成达卡他韦二盐酸盐和 merbromin 试剂之间的二元复合物。在λ _max = 544 nm处以分光光度法测量二元复合物。分光荧光法依赖于达卡他韦二盐酸盐对 merbromin 在λ_发射时的荧光强度的淬灭作用= 545 纳米。RRS 方法依赖于加入 daclatasvir dihydrochloride 后 363 nm 处 merbromin RRS 光谱的增强。所提出的方法在 2.5-15.0、0.2-1.6 和 0.15-3.0 μg ml ^-1的浓度范围内呈线性，检测限为0.45、0.046和 0.036 μg ml ^-1分别为分光光度法、分光荧光法和 RRS 法。目前的方法已根据国际协调委员会的指导方针进行了验证，并实际用于评估 daclatasvir 二盐酸盐与索非布韦的二元混合物或其商业片剂剂型，结果良好。此外，使用当前的光谱方法成功地将含量均匀性测试应用于商业片剂。

更新日期：2021-06-03

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