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External modulation of Rotimer exudate secretion in monogonant rotifers
Ecotoxicology and Environmental Safety ( IF 6.8 ) Pub Date : 2021-06-03 , DOI: 10.1016/j.ecoenv.2021.112399
Evelin Balazs 1 , Zita Galik-Olah 1 , Bence Galik 2 , Ferenc Somogyvari 3 , Janos Kalman 1 , Zsolt Datki 1
Affiliation  

The Rotimer, a rotifer-specific biopolymer, is an exogenic bioactive exudate secreted by different monogonant species (e.g. Euchlanis dilatata or Lecane bulla). The production of this viscoelastic biomolecule is induced by different micro-particles, thereby forming a special Rotimer-Inductor Conglomerate (RIC) in a web format. In this case, the water insoluble Carmine crystals, filtered to size (max. diameter was 50 µm), functioned as an inductor. The RIC production is an adequate empirical indicator to follow up this filamentous biopolymer secretion experientially; moreover, this procedure is very sensitive to the environmental factors (temperature, pH, metals and possible natural pollutant agents). The above mentioned species show completely different reactions to these factors, except to the presence of calcium and to the modulating effects of different drugs. One of the novelties of this work is that the Rotimer secretion and consequently, the RIC-formation is a mutually obligatory and evolutionary calcium-dependent process in the concerned monogonants. This in vivo procedure needs calcium, both for the physiology of animals and for fiber formation, particularly in the latter case. The conglomerate covered area (%) and the detection of the longest filament (mm) of the given RIC were the generally and simultaneously applied methods in the current modulating experiments. Exploring the regulatory (e.g. calcium-dependency) and stimulating (e.g. Lucidril effect) possibilities of biopolymer secretion are the basis for optimizing the RIC-production capacities of these micro-metazoans.



中文翻译:

单角轮虫轮虫分泌物分泌的外部调节

Rotimer 是一种轮虫特异性生物聚合物,是一种外源性生物活性渗出液,由不同的单角体物种(例如Euchlanis dilatataLecanebula)。这种粘弹性生物分子的产生是由不同的微粒诱导产生的,从而形成一种特殊的 Rotimer-Inductor Conglomerate (RIC) 网络格式。在这种情况下,将水不溶性胭脂红晶体过滤到一定尺寸(最大直径为 50 µm),起到感应器的作用。RIC 的产生是一个足够的经验指标,可以从经验上跟踪这种丝状生物聚合物的分泌;此外,该程序对环境因素(温度、pH、金属和可能的天然污染物)非常敏感。除了钙的存在和不同药物的调节作用外,上述物种对这些因素表现出完全不同的反应。这项工作的新颖之处之一是 Rotimer 分泌物,因此,RIC 形成是相关单角体中相互强制和进化的钙依赖性过程。这体内过程需要钙,用于动物生理和纤维形成,特别是在后一种情况下。砾岩覆盖面积(%)和给定RIC的最长长丝(mm)的检测是当前调制实验中普遍且同时应用的方法。探索生物聚合物分泌的调节(例如钙依赖性)和刺激(例如 Lucidril 效应)的可能性是优化这些微型后生动物 RIC 生产能力的基础。

更新日期:2021-06-03
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