Cassava pulp (CP), a by-product of the tapioca starch industry, has been recognized as a high potential substrate for bio-gas production due to its high carbon content. In this work, co-digestion between CP and distiller stillage (DS) was investigated with the main objective to improve the system stability as well as to enhance the biogas production. The effect of five different CP:DS ratios including 1:0, 1:0.5, 1:1, 0.5:1, and 0:1 based on volatile solids (VS) was carried out. The result showed that the co-digestion of CP and DS is a promising approach for increasing the cumulative methane yield by 65.57%–222.19% compared to the digestion of CP alone. The highest methane yield of 685.10 ml/g VS was obtained at the CP:DS of 1:1. The bacterial and archaeal communities were analyzed by denaturing gradient gel electrophoresis. The bacterial community of CP:DS ratio was dominated by Bacteroidetes, Firmicutes, and Chloroflexi phylum whilst Methanosarcina (Methanosarcina barkeri) dominated the methanogenic archaeal community. This work demonstrated the adaptation of co-digestion resulting in a higher methane production with a higher stability of the system. The result could pave a way for a highly efficient co-digestion system in a larger scale biogas production.

中文翻译：

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木薯浆和酒糟液共消化微生物群落结构及其生产生物甲烷的潜力研究

木薯浆 (CP) 是木薯淀粉工业的副产品，由于其高碳含量，已被公认为生物气生产的高潜力底物。在这项工作中，研究了 CP 和蒸馏器釜馏物 (DS) 之间的共同消化，主要目的是提高系统稳定性并提高沼气产量。进行了基于挥发性固体 (VS) 的五种不同 CP:DS 比率的影响，包括 1:0、1:0.5、1:1、0.5:1 和 0:1。结果表明，与单独消化 CP 相比，CP 和 DS 的共同消化是一种有前景的方法，可将累积甲烷产率提高 65.57%–222.19%。在 CP:DS 为 1:1 时获得了最高的甲烷产量 685.10 ml/g VS。通过变性梯度凝胶电泳分析细菌和古菌群落。Methanosarcina ( Methanosarcina barkeri ) 在产甲烷古菌群落中占主导地位。这项工作证明了共同消化的适应性，导致更高的甲烷产量和更高的系统稳定性。结果可以为大规模沼气生产中的高效共消化系统铺平道路。