To have therapeutic promise of neural stem/precursor cells (NS/PCs) an appropriate scaffold is mostly essential. This study was conducted to fabricate collagen (Col)/chitosan-functionalized graphene oxide (CSGO) nanocomposite hydrogel and evaluated it as scaffold for NS/PCs. Graphene oxide was first functionalized with chitosan and the obtained CSGO was then added to Col solution and the solution underwent hydrogel formation. GO sheets were exfoliated after CS functionalization and the CSGO was homogenously dispersed in Col hydrogel. CSGO addition resulted in hydrogels with higher porosity and smaller Col fibers. Furthermore, CSGO increased the gelation time and water absorption capacity while the degradation was decreased. Cell studies demonstrated higher viability of NS/PCs on Col/CSGO hydrogel comparing with Col and poly-l-lysine as control (Cnt). NS/PCs were also penetrated into the Col/CSGO hydrogel and showed more cell spreading, neurite outgrowth and inter-cell connections in comparison with Col hydrogel. In addition, the cells traveled longer distance on Col/CSGO hydrogels than on Col and Cnt, indicating excellent migration capacity of NS/PCs on Col/CSGO hydrogel. Our results indicate the potential Col/CSGO hydrogels as an appropriate scaffold for NS/PCs.

为了获得神经干/前体细胞 (NS/PC) 的治疗前景，一个合适的支架是必不可少的。本研究旨在制备胶原蛋白 (Col)/壳聚糖功能化氧化石墨烯 (CSGO) 纳米复合水凝胶，并将其作为 NS/PC 的支架进行评估。首先用壳聚糖功能化氧化石墨烯，然后将获得的 CSGO 添加到 Col 溶液中，溶液形成水凝胶。CS功能化后GO片被剥离，CSGO均匀分散在Col水凝胶中。CSGO 添加导致水凝胶具有更高的孔隙率和更小的 Col 纤维。此外，CSGO 增加了凝胶时间和吸水能力，同时减少了降解。细胞研究表明，与作为对照 (Cnt) 的 Col 和聚-l-赖氨酸相比，Col/CSGO 水凝胶上的 NS/PC 具有更高的活力。NS/PCs 也渗透到 Col/CSGO 水凝胶中，与 Col 水凝胶相比，显示出更多的细胞扩散、神经突生长和细胞间连接。此外，细胞在 Col/CSGO 水凝胶上比在 Col 和 Cnt 上移动的距离更长，表明 NS/PCs 在 Col/CSGO 水凝胶上具有出色的迁移能力。我们的结果表明潜在的 Col/CSGO 水凝胶可作为 NS/PC 的合适支架。表明 NS/PCs 在 Col/CSGO 水凝胶上具有出色的迁移能力。我们的结果表明潜在的 Col/CSGO 水凝胶可作为 NS/PC 的合适支架。表明 NS/PCs 在 Col/CSGO 水凝胶上具有出色的迁移能力。我们的结果表明潜在的 Col/CSGO 水凝胶可作为 NS/PC 的合适支架。