Abstract

For the rapid control of the highly toxic phycotoxin microcystin-LR in seafood, immunochromatographic test systems have been developed and compared using two types of gold nanoparticles as markers, namely, spherical particles and nanoflowers. The test systems are based on an indirect competitive assay, in which the detectable immune complexes include native microcystin-LR-specific antibodies and nanoparticle-labeled anti-species antibodies. The developed test systems allow for the detection of microcystin-LR within 18 min with instrumental detection limits of 0.2 and 0.1 ng/mL when using spherical nanoparticles and gold nanoflowers, respectively. The visual detection limit is 1 ng/mL for both formats. The parameters of nanoparticles, which determine the analytical characteristics of the corresponding test systems are characterized. The applicability of the developed test systems for the control of contamination of fish and seafood (shrimp, octopus, squid, and mussels) combining rapid stages of sample preparation and immunochromatography has been shown.

中文翻译：

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用于检测海鲜中微囊藻毒素-LR 的免疫色谱测试系统

摘要

为了快速控制海产品中剧毒藻毒素微囊藻毒素-LR，开发了免疫层析检测系统，并使用两种类型的金纳米粒子作为标记，即球形颗粒和纳米花进行比较。测试系统基于间接竞争分析，其中可检测的免疫复合物包括天然微囊藻毒素-LR 特异性抗体和纳米颗粒标记的抗物种抗体。当使用球形纳米粒子和金纳米花时，开发的测试系统允许在 18 分钟内检测微囊藻毒素-LR，仪器检测限分别为 0.2 和 0.1 ng/mL。两种形式的视觉检测限均为 1 ng/mL。表征确定相应测试系统的分析特性的纳米颗粒的参数。