Abstract

The production conditions were optimized, and the characteristics of biodegradable chitosan-gelatin films crosslinked with various microbial transglutaminases (transglutaminase 1, which contains maltodextrin, and transglutaminase 2, which contains maltodextrin, a milk protein) were studied. The film properties depended on both the cross-linking conditions and the type and ratio of components in the mixture. Gels and films prepared from chitosan and gelatin with transglutaminase 1 had very low characteristics as compared to films obtained with transglutaminase 2. Infrared spectroscopy, X-ray tomography, and scanning atomic force electron microscopy showed that transglutaminase 2 forms cross-links between both gelatin and chitosan amino groups, which improves the film characteristics. Films prepared from chitosan, gelatin, and glycerol crosslinked with transglutaminase 2 had the following characteristics: a strength of 15.8 MPa, a tensile strength of 220.0%, a thickness of 73.1 μm, and a density of 1.9 g/cm³. The biodegradability of the films depended on the composition and ranged from 20 to 30 days. The resulting films can serve as a matrix to obtain medical materials with antibacterial and wound-healing properties.

中文翻译：

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转谷氨酰胺酶对壳聚糖明胶薄膜性能的影响

摘要

优化了生产条件，研究了与各种微生物转谷氨酰胺酶（转谷氨酰胺酶 1，含有麦芽糊精，转谷氨酰胺酶 2，含有麦芽糊精，一种牛奶蛋白）交联的可生物降解壳聚糖-明胶薄膜的特性。薄膜性能取决于交联条件和混合物中组分的类型和比例。与使用转谷氨酰胺酶 2 获得的薄膜相比，由壳聚糖和明胶与转谷氨酰胺酶 1 制备的凝胶和薄膜具有非常低的特性。红外光谱、X 射线断层扫描和扫描原子力电子显微镜显示转谷氨酰胺酶 2 在明胶和明胶之间形成交联。壳聚糖氨基，可改善薄膜特性。由壳聚糖、明胶、³ . 薄膜的生物降解性取决于组成，范围为 20 至 30 天。所得薄膜可作为基质获得具有抗菌和伤口愈合特性的医疗材料。