ABSTRACT

Ginseng, a commonly used medicinal herb, contains polysaccharide as one of the bioactive compounds. This study aimed at evaluating the immune enhancement effects of Korean ginseng berry polysaccharides (GBPs) on macrophage RAW264.7 cells and their chemical characteristics. The crude polysaccharide (GBP-C) was extracted and fractionated to different polysaccharides (GBP-F1, GBP-F2, and GBP-F3). GBPs were mainly composed of rhamnose, arabinose, mannose, glucose and galactose, with molecular weights (M_w) ranging from 16 × 10³ to 328 × 10³ g/mol. While all the GBPs significantly increased the NO production in a dose-dependent manner, GBP-F2 showed the highest immune-enhancing effects. GBP-F2 up-regulated the mRNA expression of iNOS, IL-1β, IL-6, IL-10, and TNF-α through activating phosphorylation of NF-κB-p65, ERK1/2, JNK, and p38 to a greater extent than the other GBPs samples. These results indicate that GBPs, and especially GBP-F2, have strong immune enhancement effects desirable for application in the food and pharmaceutical industries.

摘要

人参是一种常用的药材，含有多糖作为生物活性化合物之一。本研究旨在评估高丽参浆果多糖 (GBPs) 对巨噬细胞 RAW264.7 细胞的免疫增强作用及其化学特性。粗多糖 (GBP-C) 被提取并分级为不同的多糖 (GBP-F1、GBP-F2 和 GBP-F3)。GBPs主要由鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖组成，分子量（M _w）范围为16×10 ³至328×10 ³ g/mol。虽然所有 GBP 都以剂量依赖性方式显着增加 NO 的产生，但 GBP-F2 显示出最高的免疫增强作用。GBP-F2 上调iNOS的 mRNA 表达、IL-1β、IL-6、IL-10和TNF-α通过激活 NF-κB-p65、ERK1/2、JNK 和 p38 的磷酸化在更大程度上比其他 GBP 样品。这些结果表明，GBPs，尤其是GBP-F2，具有很强的免疫增强作用，适用于食品和制药行业。