The ACC is an important brain area for the processing of pain-related information. Studies of synaptic connections within the ACC provide an understanding of basic cellular and molecular mechanisms for brain functions such as pain, emotion and related cognitive functions. Previous study of ACC synaptic transmission mainly focused on presumably thalamic inputs into pyramidal cells. In the present study, we developed a new mapping technique by combining single neuron whole-cell patch-clamp recording with 64 multi-channel field potential recording (MED64) to examine the properties of excitatory inputs into a single neuron in the ACC. We found that a single patched pyramidal neuron or interneuron simultaneously received heterogeneous excitatory synaptic innervations from different subregions (ventral, dorsal, deep, and superficial layers) in the ACC. Conduction velocity is faster as stimulation distance increases in pyramidal neurons. Fast-spiking interneurons (FS-IN) show slower inactivation when compared to pyramidal neurons and regular-spiking interneurons (RS-IN) while pyramidal neurons displayed the most rapid activation. Bath application of non-competitive AMPA receptor antagonist GYKI 53655 followed by CNQX revealed that both FS-INs and RS-INs have AMPA and KA mediated components. Our studies provide a new strategy and technique for studying the network of synaptic connections.

中文翻译：

---

成年小鼠前扣带皮层单个皮质锥体细胞或中间神经元的多个突触连接


ACC 是处理疼痛相关信息的重要大脑区域。对 ACC 内突触连接的研究有助于了解疼痛、情绪和相关认知功能等大脑功能的基本细胞和分子机制。先前对 ACC 突触传递的研究主要集中在丘脑对锥体细胞的输入上。在本研究中，我们开发了一种新的绘图技术，将单神经元全细胞膜片钳记录与 64 多通道场电位记录 (MED64) 相结合，以检查 ACC 中单个神经元的兴奋性输入的特性。我们发现单个补丁锥体神经元或中间神经元同时接收来自 ACC 不同亚区域（腹侧、背侧、深层和浅层）的异质兴奋性突触神经支配。锥体神经元的传导速度随着刺激距离的增加而加快。与锥体神经元和规则尖峰中间神经元 (RS-IN) 相比，快速尖峰中间神经元 (FS-IN) 显示出较慢的失活，而锥体神经元则显示出最快速的激活。非竞争性 AMPA 受体拮抗剂 GYKI 53655 和 CNQX 的沐浴应用表明 FS-IN 和 RS-IN 均具有 AMPA 和 KA 介导的成分。我们的研究为研究突触连接网络提供了新的策略和技术。