Recombinant adenoviruses have broad applications for gene delivery and expression. Furthermore, the adenovirus packaging system facilitates the expression of RNA-guided CRISPR/Cas9 nuclease complexes. In this study, we developed a novel system, named AdBlue, for the construction of recombinant adenoviruses using an enzymatic assembly strategy. This system could significantly reduce the time and labor required to generate adenoviral vectors. When applied to CRISPR/Cas9 design, it simplifies the preparation of recombinant adenoviruses carrying nuclease complexes and can induce high levels of site-specific mutagenesis. Our system has outstanding advantages for adenovirus preparation and could be a useful molecular engineering tool for gene delivery and editing.

中文翻译：

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一种简单快速腺病毒载体构建的新系统，以促进 CRISPR/Cas9 介导的基因组编辑


重组腺病毒在基因递送和表达方面具有广泛的应用。此外，腺病毒包装系统有利于RNA引导的CRISPR/Cas9核酸酶复合物的表达。在这项研究中，我们开发了一种名为 AdBlue 的新型系统，用于使用酶促组装策略构建重组腺病毒。该系统可以显着减少生成腺病毒载体所需的时间和劳动力。当应用于 CRISPR/Cas9 设计时，它简化了携带核酸酶复合物的重组腺病毒的制备，并且可以诱导高水平的定点诱变。我们的系统在腺病毒制备方面具有突出的优势，可以成为基因传递和编辑的有用的分子工程工具。