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Maize tissue culture, transformation, and genome editing
In Vitro Cellular & Developmental Biology - Plant ( IF 2.2 ) Pub Date : 2021-06-02 , DOI: 10.1007/s11627-021-10196-y
Albert P. Kausch , Kimberly Nelson-Vasilchik , Michael Tilelli , Joel P. Hague

The importance of maize (Zea mays L.) to global agriculture, world economy, and food security is widely known and increasing. Current maize breeding programs are deeply integrated with recent and rapid technological advances in genome sequencing, computational biology, and new genotyping and phenotyping technologies. Transformation and genome editing capabilities are a central hub to an array of advanced molecular and breeding approaches to crop improvement. Tissue culture and somatic embryogenesis play essential and central roles in maize transformation biology. Synergistic applications of maize transformation, advanced genomics, and genome editing provide a potent interdependent triad for functional genomics research and advanced molecular breeding. Implementation of advanced capabilities to transform maize and conduct genome editing will profoundly influence the dynamics of global agriculture ushering in a new era of varietal development and molecular breeding. With over 60.9 Mha planted in 2019 alone, biotech maize accounts for 31% of the world’s maize production. Up to 10% higher yields are achieved using new varieties generated using genetic modification technologies compared to similar conventional varieties. By extension, the impact new varietal releases developed through genome editing will likely be more significant. Advances in transformation and genome editing technologies will facilitate an even wider applicability for the development of new varieties with increasingly complex traits. The introduction of biochemical pathways and the use of synthetic biology have become increasingly more attainable. The future is of genotype independent maize transformation and genome editing, as the working platform will impact world agriculture, global food security, and plant science well into the future.



中文翻译:

玉米组织培养、转化和基因组编辑

玉米的重要性(Zea maysL.) 对全球农业、世界经济和粮食安全的影响广为人知且日益增加。当前的玉米育种计划与基因组测序、计算生物学以及新的基因分型和表型技术方面最近的快速技术进步深度融合。转化和基因组编辑能力是一系列用于作物改良的先进分子和育种方法的中心枢纽。组织培养和体细胞胚胎发生在玉米转化生物学中起着重要和核心的作用。玉米转化、先进基因组学和基因组编辑的协同应用为功能基因组学研究和先进分子育种提供了强大的相互依赖的三联体。转化玉米和进行基因组编辑的先进能力的实施将深刻影响全球农业的动态,开创品种开发和分子育种的新时代。仅 2019 年就种植了超过 60.9 Mha,生物技术玉米占世界玉米产量的 31%。与类似的传统品种相比,使用基因改造技术产生的新品种可将产量提高 10%。通过扩展,通过基因组编辑开发的新品种发布的影响可能会更显着。转化和基因组编辑技术的进步将有助于更广泛地开发具有日益复杂性状的新品种。生化途径的引入和合成生物学的使用变得越来越容易实现。未来是基因型独立的玉米转化和基因组编辑,因为该工作平台将影响未来的世界农业、全球粮食安全和植物科学。

更新日期:2021-06-02
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