H₂S is well-known as a colorless, acidic gas, with a notoriously rotten-egg smell. It was recently revealed that H₂S is also an endogenous signaling molecule that has important biological functions, however, most of its physiology and pathology remains elusive. Therefore, the enthusiasm for H₂S research remains. Fluorescence imaging technology is an important tool for H₂S biology research. The development of fluorescence imaging technology has realized the study of H₂S in subcellular organelles, facilitated by the development of fluorescent probes. The probes reviewed in this paper were categorized according to their chemical mechanism of sensing and were divided into three groups: H₂S reducibility-based probes, H₂S nucleophilicity-based probes, and metal sulfide precipitation-based probes. The structure of the probes, their sensing mechanism, and imaging results have been discussed in detail. Moreover, we also introduced some probes for hydrogen polysulfides.

H ₂ S 是众所周知的无色酸性气体，具有臭名昭著的臭鸡蛋味。最近发现，H ₂ S 也是一种具有重要生物学功能的内源性信号分子，然而，其大部分生理学和病理学仍然难以捉摸。因此，对H ₂ S 研究的热情依然存在。荧光成像技术是H ₂ S生物学研究的重要工具。随着荧光探针的发展，荧光成像技术的发展实现了对亚细胞器中H ₂ S的研究。本文综述的探针根据其传感化学机制分为三类： H ₂基于 S 还原性的探针、基于 H ₂ S 亲核性的探针和基于金属硫化物沉淀的探针。详细讨论了探针的结构、传感机制和成像结果。此外，我们还介绍了一些针对多硫化氢的探针。