Weaning is one of the most stressful events in the pig's life, which disrupts physiological balance and leads to oxidative stress. It is reported that glucose oxidase supplementation could alleviate oxidative stress in animals by increasing the concentration of antioxidant enzymes in vivo. The purpose of this study was to evaluate the effects of dietary supplementation of Aspergillus niger-expressed glucose oxidase (AN-GOX) on growth performance, nutrient digestibility, faecal microbiota, faecal gas emission and serum antioxidant enzyme parameters in weaning pigs. A total of 120 21-day-old weaning pigs [(Yorkshire ×Landrace) × Duroc] with an initial body weight of 6.54 ± 0.55 kg were used in a 21-day experiment (phase 1, days 1–7; phase 2, days 8–21) with a completely randomized block design. Pigs were randomly divided into 4 treatment groups with 6 replicate pens per treatment and 5 pigs per pen (2 barrows and 3 gilts). Dietary treatments were corn-soybean meal-based basal diet supplemented with 0, 0.01, 0.03 or 0.05% AN-GOX (1000 unit/g). The results of this study showed that average daily gain during days 1–7 and 1–21 and the concentrations of serum glutathione peroxidase and glutathione increased linearly at graduated doses of AN-GOX increased in the diet. However, dietary supplementation of AN-GOX had no effects on the apparent nutrient digestibility, faecal microbiota and faecal gas emission. In conclusion, supplementing AN-GOX to the diet of weaning pigs ameliorated weaning stress, which manifested as the increase in serum antioxidant enzyme levels, thus improving growth performance. The suitable dosage of AN-GOX used in the diet of weaning pigs was 0.05%.

中文翻译：

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日粮添加黑曲霉表达的葡萄糖氧化酶可改善断奶应激并改善断奶猪的生长性能

断奶是猪一生中压力最大的事件之一，它会破坏生理平衡并导致氧化应激。据报道，补充葡萄糖氧化酶可以通过增加体内抗氧化酶的浓度来缓解动物的氧化应激。本研究的目的是评估膳食补充剂黑曲霉的效果-表达的葡萄糖氧化酶 (AN-GOX) 对断奶猪的生长性能、养分消化率、粪便微生物群、粪便气体排放和血清抗氧化酶参数的影响。在为期 21 天的实验（第 1 阶段，第 1-7 天；第 2 阶段，第 8-21 天）采用完全随机区组设计。猪被随机分为 4 个处理组，每个处理 6 个重复栏，每栏 5 头猪（2 头公猪和 3 头后备母猪）。日粮处理为以玉米-豆粕为基础的基础日粮，辅以 0、0.01、0.03 或 0.05% AN-GOX（1000 单位/克）。这项研究的结果表明，在日粮中逐渐增加的 AN-GOX 剂量增加时，第 1-7 天和第 1-21 天的平均日增重以及血清谷胱甘肽过氧化物酶和谷胱甘肽的浓度线性增加。然而，日粮中添加 AN-GOX 对表观养分消化率、粪便微生物群和粪便气体排放没有影响。总之，在断奶仔猪日粮中添加AN-GOX可改善断奶应激，表现为血清抗氧化酶水平升高，从而改善生长性能。断奶仔猪日粮中AN-GOX的适宜用量为0.05%。