Primary open-angle glaucoma (POAG) is tightly related with extracellular matrix (ECM) remodeling of human trabecular meshwork cells (HTMCs). Transforming growth factor-β2 (TGF-β2) can induce ECM remodeling. The aim of the study was to investigate the microRNAs (miRNAs) expression changes of extracellular vesicles (EVs) derived from HTMCs treated with TGF-β2. EVs were isolated from HTMCs supernatant cultured for 24 h with TGF-β2. The morphology of EVs pellets was examined by transmission electron microscopy. Nanoparticle tracking analysis used to demonstrate the particle size distribution. Total EVs RNAs were extracted for subsequent miRNA gene chip analysis to investigate differentially expressed miRNAs between the controls and treatment cells. Gene Ontology (GO) annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were used to predict potential target and validate possible functions of the miRNAs. There were 23 miRNAs upregulated and 3 miRNAs downregulated and 469,102, and 94 GO terms involved in biological processes, cellular components, and molecular function for the possible functions of the 26 miRNAs. These findings indicate that TGF-β2 may alter EVs miRNAs expression to participate in the pathogenesis of POAG. They may provide significant information for potential biomarkers for POAG diagnosis and treatment.

中文翻译：

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转化生长因子-β2 诱导的原代人小梁网细胞胞外囊泡 miRNA 的表达改变

原发性开角型青光眼 (POAG) 与人小梁网细胞 (HTMC) 的细胞外基质 (ECM) 重塑密切相关。转化生长因子-β2 (TGF-β2) 可以诱导 ECM 重塑。该研究的目的是研究来自用 TGF-β2 处理的 HTMCs 的细胞外囊泡 (EVs) 的 microRNAs (miRNAs) 表达变化。从用 TGF-β2 培养 24 小时的 HTMC 上清液中分离出 EV。通过透射电子显微镜检查 EV 颗粒的形态。用于证明粒径分布的纳米粒子追踪分析。提取总 EV RNA 用于随后的 miRNA 基因芯片分析，以研究对照和治疗细胞之间差异表达的 miRNA。基因本体（GO）注释，京都基因和基因组百科全书 (KEGG) 通路富集分析用于预测潜在目标并验证 miRNA 的可能功能。有 23 个 miRNAs 上调，3 个 miRNAs 下调，469,102 和 94 个 GO 术语涉及 26 个 miRNA 的可能功能的生物过程、细胞成分和分子功能。这些发现表明 TGF-β2 可能改变 EVs miRNAs 的表达以参与 POAG 的发病机制。它们可能为 POAG 诊断和治疗的潜在生物标志物提供重要信息。以及 26 种 miRNA 可能功能的分子功能。这些发现表明 TGF-β2 可能改变 EVs miRNAs 的表达以参与 POAG 的发病机制。它们可能为 POAG 诊断和治疗的潜在生物标志物提供重要信息。以及 26 种 miRNA 可能功能的分子功能。这些发现表明 TGF-β2 可能改变 EVs miRNAs 的表达以参与 POAG 的发病机制。它们可能为 POAG 诊断和治疗的潜在生物标志物提供重要信息。