BACKGROUND:Andean berry contains several classes of phenolic compounds which have showed antioxidant and cytotoxic activity. OBJECTIVE:To characterize the Andean berry aqueous extract and to study their anti-proliferative mechanisms on SW480 and SW620 cell lines (human colon adenocarcinoma). METHODS:Total phenolic and total anthocyanins contents were determined by Folin-Ciocalteau and differential pH methods, respectively. Antioxidant activity was measured by FRAP, ORAC and DPPH methods. Antiproliferative effect was determined by sulforhodamine colorimetric method and apoptosis was analyzed by flow cytometry using propidium iodide/Annexin-V. Mitochondrial potential was evaluated using DIOC6 and ROS levels were measured by 2,7-Dicholorodihydrofluorescein diacetate (DCFH-DA). RESULTS:The total phenol and anthocyanin content were 4409.78± 63,05 mg equivalents of gallic acid/100 mL and 106,57± 1.43 mg equivalents of cyanidin-3-glycoside/100 mL, respectively. Andean berry extract showed antioxidant activity by FRAP, ORAC and DPPH methods and antiproliferative effect on SW480 and SW620 cells. It was observed a cell cycle arrest at S and G2/M phases on SW480 and at G0/G1 phase on SW620 cells. Aqueous extract did not induce mitochondrial depolarization or affect intracellular ROS levels. CONCLUSIONS:Andean berry aqueous extract has antioxidant capacity and induces apoptosis involving cell cycle arrest in SW480 and SW620 cells without mitochondrial damage.

中文翻译：

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安第斯浆果水提物诱导人结肠癌细胞凋亡而不损伤线粒体

背景：安第斯浆果含有几类酚类化合物，具有抗氧化和细胞毒活性。目的：表征安第斯浆果水提物并研究其对 SW480 和 SW620 细胞系（人结肠腺癌）的抗增殖机制。方法：分别采用Folin-Ciocalteau法和微分pH法测定总酚类和总花青素含量。通过FRAP、ORAC和DPPH方法测量抗氧化活性。通过磺基罗丹明比色法测定抗增殖作用，并使用碘化丙啶/膜联蛋白-V 通过流式细胞术分析细胞凋亡。使用 DIOC6 评估线粒体电位，并通过 2,7-二氯二氢荧光素二乙酸酯 (DCFH-DA) 测量 ROS 水平。结果：总酚和花青素含量为4409.78±63，分别为 05 mg 没食子酸/100 mL 和 106,57± 1.43 mg 当量的 cyanidin-3-glycoside/100 mL。安第斯浆果提取物通过 FRAP、ORAC 和 DPPH 方法显示出抗氧化活性以及对 SW480 和 SW620 细胞的抗增殖作用。观察到 SW480 细胞在 S 期和 G2/M 期以及 SW620 细胞在 G0/G1 期出现细胞周期停滞。水提取物不会诱导线粒体去极化或影响细胞内 ROS 水平。结论：安第斯浆果水提取物具有抗氧化能力，可诱导 SW480 和 SW620 细胞凋亡，涉及细胞周期停滞，而没有线粒体损伤。ORAC 和 DPPH 方法以及对 SW480 和 SW620 细胞的抗增殖作用。观察到 SW480 细胞在 S 期和 G2/M 期以及 SW620 细胞在 G0/G1 期出现细胞周期停滞。水提取物不会诱导线粒体去极化或影响细胞内 ROS 水平。结论：安第斯浆果水提取物具有抗氧化能力，可诱导 SW480 和 SW620 细胞凋亡，涉及细胞周期停滞，而没有线粒体损伤。ORAC 和 DPPH 方法以及对 SW480 和 SW620 细胞的抗增殖作用。观察到 SW480 细胞在 S 期和 G2/M 期以及 SW620 细胞在 G0/G1 期出现细胞周期停滞。水提取物不会诱导线粒体去极化或影响细胞内 ROS 水平。结论：安第斯浆果水提取物具有抗氧化能力，可诱导 SW480 和 SW620 细胞凋亡，涉及细胞周期停滞，而没有线粒体损伤。