BACKGROUND:In blueberries, the total polyphenol contents, total anthocyanin contents, and antioxidant activities of fruits in blueberries and their relatives are becoming important targets for breeders. Recently, intersectional hybrids between Japanese wild species Shashanbo and highbush blueberrywere produced using polyploid breeding for the first time. However, the polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties of the fruits of these intersectional hybrids have not been studied sufficiently. OBJECTIVE:The objectives of this study were to determine the anthocyanin contents, polyphenol contents, and antioxidant activities at each stage of fruit development in intersectional hybrids, and to examine the effects of the fruit extracts on anti-cancer cell proliferation properties using human promyelocytic leukemia (HL-60) cells. METHODS:Freeze-dried fruit samples of three intersectional hybrids and their parents at five developmental stages were used to evaluate their polyphenol contents, anthocyanin contents, and levels of two types of antioxidant activities (DPPH and ORAC). The anti-cancer cell proliferation properties of each extract were also evaluated according to the survival rate of HL-60 cells. RESULTS:The total polyphenol contents in fruits of the intersectional hybrids gradually decreased as the fruits grew, but the contents increased slightly at the mature stage. Meanwhile, the total anthocyanin contents increased strongly to the mature stage. In addition, the fruit extract of intersectional hybrid JM1 was found to be more effective for inhibiting the growth of HL-60 human leukemia cells in vitro. Total polyphenol content correlated highly with antioxidant activities and anti-cancer cell proliferation properties. CONCLUSIONS:We clarified that the intersectional hybrids had produced have abundant bioactive compounds, high levels of antioxidant activities, and anti-cancer cell proliferation properties. The results proved the effectiveness of the use of Japanese wild Vaccinium species for the strategic breeding of blueberries with high levels of bioactive compounds.

中文翻译：

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高丛蓝莓与沙山波（Vaccinium bracteatum Thunb.）交叉杂交果实发育各个阶段的多酚含量、抗氧化活性和抗癌细胞增殖特性。

背景：在蓝莓中，蓝莓及其近缘果实的总多酚含量、总花青素含量和抗氧化活性正成为育种者关注的重要指标。最近，日本野生物种沙山伯与高丛蓝莓的交叉杂交首次通过多倍体育种产生。然而，这些交叉杂种果实的多酚含量、抗氧化活性和抗癌细胞增殖特性尚未得到充分研究。目的：本研究的目的是测定交叉杂种果实发育各个阶段的花青素含量、多酚含量和抗氧化活性。并使用人早幼粒细胞白血病 (HL-60) 细胞检查水果提取物对抗癌细胞增殖特性的影响。方法：采用冷冻干燥的3个杂交杂交种及其亲本5个发育阶段的果实样品，评估其多酚含量、花青素含量和两种抗氧化活性（DPPH和ORAC）水平。还根据HL-60细胞的存活率评估了每种提取物的抗癌细胞增殖特性。结果：交叉杂种果实的总多酚含量随着果实的生长而逐渐降低，但在成熟期略有增加。同时，到成熟期，总花青素含量显着增加。此外，发现交叉杂交 JM1 的果实提取物在体外抑制 HL-60 人白血病细胞的生长方面更有效。总多酚含量与抗氧化活性和抗癌细胞增殖特性高度相关。结论：我们阐明所产生的交叉杂种具有丰富的生物活性化合物、高水平的抗氧化活性和抗癌细胞增殖特性。结果证明了使用日本野生越桔物种对具有高水平生物活性化合物的蓝莓进行战略育种的有效性。我们澄清，产生的交叉杂种具有丰富的生物活性化合物、高水平的抗氧化活性和抗癌细胞增殖特性。结果证明了使用日本野生越桔物种对具有高水平生物活性化合物的蓝莓进行战略育种的有效性。我们澄清，产生的交叉杂种具有丰富的生物活性化合物、高水平的抗氧化活性和抗癌细胞增殖特性。结果证明了使用日本野生越桔物种对具有高水平生物活性化合物的蓝莓进行战略育种的有效性。