BACKGROUND:The prevention of rheologic alterations in erythrocytes may be important for reducing sepsis-associated morbidity and mortality. Remote ischemic preconditioning (RIPC) has been shown to prevent tissue damage caused by severe ischemia and mortality resulting from sepsis. However, the effect of RIPC on erythrocytes in sepsis is yet to be determined. OBJECTIVE:To investigate the effect of RIPC on rheologic alterations in erythrocytes in sepsis. METHODS:Thirty male Sprague-Dawley rats were used in this study. An endotoxin-induced sepsis model was established by intraperitoneally injecting 20 mg/kg LPS (LPS group). RIPC was induced in the right hind limb using a tourniquet, with three 10-minute of ischemia and 10 min of reperfusion cycles immediately before the injection of LPS (RIPC/LPS group) or phosphate-buffered saline (RIPC group). The aggregation index (AI), time to half-maximal aggregation (T1/2), and maximal elongation index (EImax) of the erythrocytes were measured 8 h after injection. RESULTS:The AI, T1/2, and EImax values in the LPS and RIPC/LPS groups differed significantly from those in the RIPC group, but there were no differences between the values in the LPS and RIPC/LPS groups. CONCLUSIONS:RIPC did not prevent rheologic alterations in erythrocytes in the rat model of LPS-induced endotoxemia.

中文翻译：

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远程缺血预处理对大鼠内毒素血症模型红细胞变形能力和聚集的影响

背景：预防红细胞流变学改变对于降低败血症相关的发病率和死亡率可能很重要。远程缺血预处理 (RIPC) 已被证明可以防止严重缺血引起的组织损伤和败血症导致的死亡。然而，RIPC 对脓毒症红细胞的影响尚待确定。目的：探讨RIPC对脓毒症红细胞流变学改变的影响。方法：本研究使用30只雄性Sprague-Dawley大鼠。通过腹腔注射20 mg/kg LPS（LPS组）建立内毒素诱导的败血症模型。使用止血带在右后肢诱导 RIPC，在注射 LPS（RIPC/LPS 组）或磷酸盐缓冲盐水（RIPC 组）之前立即进行 3 个 10 分钟的缺血和 10 分钟的再灌注循环。注射后 8 小时测量红细胞的聚集指数 (AI)、半最大聚集时间 (T1/2) 和最大伸长指数 (EImax)。结果：LPS和RIPC/LPS组的AI、T1/2和EImax值与RIPC组有显着差异，但LPS和RIPC/LPS组的值无差异。结论：RIPC 不能阻止 LPS 诱导的内毒素血症大鼠模型中红细胞的流变学改变。