Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar. The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe), compared with other treatments. The results also indicate that using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe) as a supplement can decrease antioxidant enzymes CAT and POD activity compared to the rest of the treatments. Medium equipped with 187.0 mg L−1 FeEDDHA (11.2 mg L−1Fe) had the highest rooting percentage and number of roots per shoot than other treatments. The biochemical analysis results showed that treatments with FeEDDHA of 280.5 mg L−1 (16.8 mg L−1 Fe) and 187.0 mg L−1 (11.2 mg L−1Fe) significantly increased the iron content. The results showed that shoot maximum chlorophyll and endogenous IAA level content were recorded in a medium supplemented with 187.0 mg L−1 FeEDDHA (11.2 mg L−1Fe) as Fe source. FeEDDHA used in the present study was proven to be a promising iron chelate source in comparison with the FeEDTA sources.

中文翻译：

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枣椰树（Phoenix dactylifera L.）体外植物再生系统：螯合铁源的影响

铁螯合物来源及其浓度是枣椰离体繁殖的重要因素。本研究的目的是研究铁螯合形式对 Barhee 品种组织培养物生长和发育的影响。在培养基中添加 FeEDDHA 在愈伤组织生长、芽再生和每罐芽数方面比 FeEDTA 更有效，其中最好的结果（220.8mg 愈伤组织，分别为 86.67% 和 17.2 个芽每罐）通过与其他处理相比，使用 93.5 mg L-1 FeEDDHA（5.6 mg L-1 Fe）。结果还表明，与其余处理相比，使用 93.5 mg L-1 FeEDDHA（5.6 mg L-1 Fe）作为补充剂可以降低抗氧化酶 CAT 和 POD 活性。配备 187.0 mg L-1 FeEDDHA (11. 2 mg L−1Fe) 的生根百分比和每个芽的根数比其他处理最高。生化分析结果表明，FeEDDHA 280.5 mg L-1（16.8 mg L-1 Fe）和187.0 mg L-1（11.2 mg L-1Fe）处理显着增加了铁含量。结果表明，在补充有 187.0 mg L-1 FeEDDHA (11.2 mg L-1Fe) 作为 Fe 源的培养基中记录了枝条最大叶绿素和内源 IAA 水平含量。与 FeEDTA 来源相比，本研究中使用的 FeEDDHA 被证明是一种很有前途的铁螯合物来源。结果表明，在补充有 187.0 mg L-1 FeEDDHA (11.2 mg L-1Fe) 作为 Fe 源的培养基中记录了枝条最大叶绿素和内源 IAA 水平含量。与 FeEDTA 来源相比，本研究中使用的 FeEDDHA 被证明是一种很有前途的铁螯合物来源。结果表明，在补充有 187.0 mg L-1 FeEDDHA (11.2 mg L-1Fe) 作为 Fe 源的培养基中记录了枝条最大叶绿素和内源 IAA 水平含量。与 FeEDTA 来源相比，本研究中使用的 FeEDDHA 被证明是一种很有前途的铁螯合物来源。