Past commercial and clinical research on wound dressings generally only focused on their therapeutic activities, however, how to make products environmentally friendly with reduced production of hazardous waste while ensuring product functionality is becoming a crucial and burning issue in the manufacturing industry. To address this problem, we developed a natural, degradable and environmentally friendly hydrogel wound dressing using aloe vera (AV) as an active ingredient. The hydrogel wound dressing was prepared using all natural ingredients, comprised of sodium hyaluronate (SH), dopamine (DA), chitosan (CS), and AV, and using a green natural deep eutectic solvent (DES) as a green solvent. The resultant DES-SH-CS/DA/AV hydrogel was easy to degrade and the preparation process was environmentally friendly. The chemical and physical properties of the hydrogel samples were characterized. The DES-SH-CS/DA/AV hydrogel showed good cytocompatibility tested using NIH-3T3 fibroblast cells, and antibacterial properties against both gram-positive (S. aureus) and gram-negative (E. coli). Furthermore, the hydrogel promoted skin tissue regeneration healed the wound of mouse skin after surgery within 12 days. The H&E staining was used to assess the integrity of the regenerated epidermis and dermis and the new generation of skin attachments in this work. Overall, our study showed the DES-SH-CS/DA/AV hydrogel we developed has the potential to be used as a natural and green wound dressing.

过去对伤口敷料的商业和临床研究通常只关注其治疗活动，然而，如何在确保产品功能的同时使产品环保、减少危险废物的产生正成为制造业中一个至关重要且亟待解决的问题。为了解决这个问题，我们开发了一种使用芦荟 (AV) 作为活性成分的天然、可降解和环保的水凝胶伤口敷料。水凝胶伤口敷料采用全天然成分制备，包括透明质酸钠 (SH)、多巴胺 (DA)、壳聚糖 (CS) 和 AV，并使用绿色天然低共熔溶剂 (DES) 作为绿色溶剂。所得DES-SH-CS/DA/AV水凝胶易降解，制备过程环保。表征了水凝胶样品的化学和物理性质。DES-SH-CS/DA/AV 水凝胶在 NIH-3T3 成纤维细胞测试中显示出良好的细胞相容性，以及对革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌）。此外，水凝胶促进皮肤组织再生，在12天内治愈了手术后小鼠皮肤的伤口。在这项工作中，H&E 染色用于评估再生表皮和真皮以及新一代皮肤附着物的完整性。总体而言，我们的研究表明，我们开发的 DES-SH-CS/DA/AV 水凝胶具有用作天然绿色伤口敷料的潜力。