Fusarium verticillioides is a phytopathogenic fungus that can contaminate maize grain silos and result in important losses in the post-harvest product. The objective of this work was to investigate the effects of volatile organic compounds produced by four lactic acid bacterial strains isolated from maize grain silos on F. verticillioides M3125 growth and fumonisin B₁ (FB₁) production. The bacterial isolates 55 and 49 were identified as Enterococcus faecium and M4A and M4G as Enterococcus casseliflavus. The fungal growth was inhibited by 33.33% by the volatiles released by the M4A strain and by approximately 10% by the volatiles emitted by the 55 and 49 strains. The volatiles produced by the M4A strain also significantly reduced (88.75%) FB₁ biosynthesis. The gas chromatography–mass spectrometer analysis identified 21 volatile organic compounds, with diacetyl, acetic acid and acetoin being the main volatiles emitted by the four bacterial strains. Acetoin was the volatile produced in the highest proportion by the four strains, with M4A generating the highest proportion of diacetyl (35.11%). Diacetyl and acetic acid completely inhibited fungal growth at concentrations of 0.3 and 1 mM, respectively, while acetoin promoted fungal growth. Only acetoin significantly reduced FB₁ production. These results showed that diacetyl was the main compound involved in fungal inhibition, while the effect on FB₁ production could have been due to the combination of the volatile organic compounds produced by the M4A strain. In conclusion, the volatiles emitted by the E. casseliflavus M4A strain could be a promising tool for the biocontrol of F. verticillioides in storage maize grain silos.

Fusarium v​​erticillioides是一种植物病原真菌，可污染玉米粮仓并导致收获后产品的重大损失。这项工作的目的是研究从玉米粮仓中分离的四种乳酸菌菌株产生的挥发性有机化合物对轮枝杆菌 M3125 生长和伏马菌素 B ₁ (FB ₁ ) 产量的影响。分离菌 55 和 49 被鉴定为屎肠球菌，M4A 和 M4G被鉴定为球状肠球菌. 真菌生长被 M4A 菌株释放的挥发物抑制了 33.33%，被 55 和 49 菌株释放的挥发物抑制了大约 10%。M4A 菌株产生的挥发物也显着降低 (88.75%) FB ₁生物合成。气相色谱-质谱分析确定了 21 种挥发性有机化合物，其中二乙酰、乙酸和乙偶姻是四种细菌菌株释放的主要挥发物。乙偶姻是四种菌株产生的比例最高的挥发物，其中 M4A 产生的双乙酰比例最高（35.11%）。二乙酰和乙酸分别在 0.3 和 1 mM 的浓度下完全抑制真菌生长，而乙偶姻促进真菌生长。只有乙偶姻显着降低了 FB ₁生产。这些结果表明双乙酰是参与真菌抑制的主要化合物，而对 FB ₁产生的影响可能是由于 M4A 菌株产生的挥发性有机化合物的组合。总之，E. casseliflavus M4A 菌株释放的挥发物可能是一种很有前途的工具，用于在玉米储粮筒仓中对轮枝杆菌进行生物防治。