Root feeding by the larvae of multiple Delia species can lead to economic loss in many agricultural crops. Field vegetables are subject to infestations by a species complex composed of Delia radicum (Linnaeus) (Diptera: Anthomyiidae), a pest in brassica crops (Brassicaceae), Delia antiqua (Meigen), believed to cause the majority of crop damage in onions, and the generalists Delia florilega (Zetterstedt), Delia platura (Meigen), and Botanophila fugax (Meigen) (Diptera: Anthomyiidae). Correct species identification is necessary to implement field management strategies, but these species are challenging to identify morphologically. We propose a polymerase chain reaction–restriction fragment length polymorphism method as a molecular tool to distinguish between five species of Delia and between two genetic lines of D. platura. The mitochondrial DNA cytochrome c oxidase subunit 1 barcode fragment is targeted, then the polymerase chain reaction product digested with four different restriction enzymes (AccI, BsrI, MlyI, and StyI). The BsrI enzyme distinguishes the two genetic lines of D. platura and D. florilega. The MlyI enzyme identifies B. fugax from the Delia species. Combining BsrI, StyI, AccI, and MlyI into double digestion reactions allows for rapid diagnostics among the species tested. Our method was validated using DNA from specimens collected in eastern Canada. This method provides tools in ecological and environmental studies where these species are of interest.

多种迪利亚幼虫以根为食会导致许多农作物的经济损失。田间蔬菜易受由菖蒲（Linnaeus）（双翅目：花螟科）组成的物种复合体的侵染，这是一种芸苔属作物（十字花科）的害虫，地螟（Meigen），据信对洋葱造成大部分作物损害，以及通才Delia florilega (Zetterstedt)、Delia platura (Meigen) 和Botanophila fugax(Meigen) (双翅目: Anthomyiidae)。正确的物种识别对于实施田间管理策略是必要的，但这些物种在形态学上的识别具有挑战性。我们提出了一种聚合酶链反应-限制性片段长度多态性方法，作为区分五种Delia物种和D. platura 的两个遗传系的分子工具。靶向线粒体 DNA 细胞色素 c 氧化酶亚基 1 条码片段，然后用四种不同的限制酶（Acc I、Bsr I、Mly I 和Sty I）消化聚合酶链反应产物。所述BSR我酶区分的两个遗传线D. platura和D. florilega。的万光年我酶识别B. fugax从迪莉娅物种。将Bsr I、Sty I、Acc I 和Mly I 结合到双消化反应中，可以对测试的物种进行快速诊断。我们的方法使用在加拿大东部收集的样本的 DNA 进行了验证。这种方法为这些物种感兴趣的生态和环境研究提供了工具。