Interleukin 10 (IL-10) is a synthetic inhibitor of human cytokines with immunomodulatory and anti-inflammatory effects. This study was designed to investigate the expression variation of IL-10 in the multiple sites including cortex, hippocampus, and lung tissues of neonatal hypoxic-ischemic (HI) rats and explore the crucial role of IL-10 in alleviating HI brain damage. In this study, neonatal Sprague-Dawley rats were subjected to the right common carotid artery ligation, followed by 2 h of hypoxia. The expression of IL-10 in the cortex, hippocampus, and lung tissues was measured with immunohistochemistry, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot (WB). Immunofluorescence double staining was performed to observe the localization of IL-10 in neurons and astrocytes. Moreover, not-targeting and targeting IL-10 siRNA lentivirus vectors were injected into the rats of the negative control (NC) and IL-10 group, respectively, and the mRNA levels of B-cell lymphoma 2 (Bcl-2) and endoplasmic reticulum protein 29 (ERp29) were detected by RT-qPCR following IL-10 silence. The results demonstrated that the IL-10 expression was markedly increased after HI and IL-10 were colocalized with neurons and astrocytes which were badly injured by HI insult. In addition, Bcl-2 and ERp29 were remarkably decreased following IL-10 mRNA interference compared with the NC group. Our findings revealed that IL-10 exerted its antiapoptotic and neuroprotective effects by regulating the expression of Bcl-2 and ERp29, indicating that IL-10 may be a promising molecule target for HIE treatment.

中文翻译：

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白介素 10 在与 B 细胞淋巴瘤 2 和内质网蛋白 29 相关的缺氧缺血新生大鼠中发挥重要作用


白细胞介素 10 (IL-10) 是一种合成的人类细胞因子抑制剂，具有免疫调节和抗炎作用。本研究旨在探讨新生缺氧缺血（HI）大鼠皮层、海马、肺组织等多个部位IL-10的表达变化，探讨IL-10在减轻HI脑损伤中的重要作用。本研究对新生Sprague-Dawley大鼠进行右侧颈总动脉结扎，随后缺氧2小时。通过免疫组织化学、实时定量聚合酶链反应（RT-qPCR）和蛋白质印迹（WB）测量皮质、海马和肺组织中IL-10的表达。免疫荧光双染观察IL-10在神经元和星形胶质细胞中的定位。分别向阴性对照（NC）组和IL-10组大鼠注射非靶向和靶向IL-10 siRNA慢病毒载体，检测B细胞淋巴瘤2（Bcl-2）和内质网的mRNA水平。 IL-10 沉默后通过 RT-qPCR 检测网状蛋白 29 (ERp29)。结果表明，HI和IL-10与因HI损伤而严重损伤的神经元和星形胶质细胞共定位后，IL-10表达显着增加。此外，与NC组相比，IL-10 mRNA干扰后Bcl-2和ERp29显着减少。我们的研究结果表明，IL-10 通过调节 Bcl-2 和 ERp29 的表达发挥抗凋亡和神经保护作用，表明 IL-10 可能是 HIE 治疗的一个有前景的分子靶点。