A novel hemostatic nanocomposite (OBC-PDA/PDA-MMT/Ag NPs) was prepared. As Functional hemostatic particles, hydrochloric acid modified montmorillonite coated with dopamine (PDA-MMT) doped into oxidized bacterial cellulose (OBC). In the presence of carboxyl and dopamine, silver ions (Ag⁺) were reduced into Ag nanoparticles (Ag NPs) distributed homogeneously on the matrix of PDA-MMT and OBC. Then, dopamine was grafted onto the oxidized bacterial cellulose under the crosslinking effect of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC). After dopamine was grafted onto the oxidized bacterial cellulose, the interaction between PDA-MMT and the whole material was enhanced, and the flexibility was also improved. OBC-PDA/PDA-MMT/Ag NPs hemostatic sponge have appropriate mechanical strength, broad-spectrum antibacterial properties and excellent biodegradability. The hemostatic sponge with addition of PDA-MMT and Ag NPs is expected to provide functional properties such as rapid hemostasis, bacteriostasis and wound healing. In addition, the hemostatic effect of the compound was confirmed in vivo. The hemostatic sponge showed greater coagulation capacity, higher adherent red blood cells and platelets, and lower blood loss. The results show that hemostatic sponge is a rapid and effective coagulant with good antibacterial properties.

制备了一种新型止血纳米复合材料（OBC-PDA/PDA-MMT/Ag NPs）。作为功​​能性止血颗粒，盐酸改性蒙脱石涂有多巴胺 (PDA-MMT)，掺杂到氧化细菌纤维素 (OBC) 中。在羧基和多巴胺存在下，银离子（Ag ⁺) 被还原成均匀分布在 PDA-MMT 和 OBC 基质上的银纳米颗粒 (Ag NPs)。然后，在 1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺 (EDC) 的交联作用下，多巴胺接枝到氧化的细菌纤维素上。在氧化细菌纤维素上接枝多巴胺后，PDA-MMT与整个材料的相互作用增强，柔韧性也得到提高。OBC-PDA/PDA-MMT/Ag NPs止血海绵具有适当的机械强度、广谱抗菌性能和优异的生物降解性。添加 PDA-MMT 和 Ag NPs 的止血海绵有望提供快速止血、抑菌和伤口愈合等功能特性。此外，该化合物的止血作用在体内得到证实。止血海绵显示出更强的凝血能力，更高的粘附红细胞和血小板，以及更低的失血量。结果表明，止血海绵是一种快速有效的凝血剂，具有良好的抗菌性能。